論文 - 詳細
| RRC ID | 59356 |
|---|---|
| 著者 | Hu Q, D'Amora DR, MacNeil LT, Walhout AJM, Kubiseski TJ. |
| タイトル | The Caenorhabditis elegans Oxidative Stress Response Requires the NHR-49 Transcription Factor. |
| ジャーナル | G3 (Bethesda) |
| Abstract |
The overproduction of reactive oxygen species (ROS) in cells can lead to the development of diseases associated with aging. We have previously shown that C. elegans BRAP-2 (Brca1 associated binding protein 2) regulates phase II detoxification genes such as gst-4, by increasing SKN-1 activity. Previously, a transcription factor (TF) RNAi screen was conducted to identify potential activators that are required to induce gst-4 expression in brap-2(ok1492) mutants. The lipid metabolism regulator NHR-49/HNF4 was among 18 TFs identified. Here, we show that knockdown of nhr-49 suppresses the activation of gst-4 caused by brap-2 inactivation and that gain-of-function alleles of nhr-49 promote gst-4 expression. We also demonstrate that nhr-49 and its cofactor mdt-15 are required to express phase II detoxification enzymes upon exposure to chemicals that induce oxidative stress. Furthermore, we show that NHR-49 and MDT-15 enhance expression of skn-1a/c These findings identify a novel role for NHR-49 in ROS detoxification by regulating expression of SKN-1C and phase II detoxification genes. |
| 巻・号 | 8(12) |
| ページ | 3857-3863 |
| 公開日 | 2018-12-10 |
| DOI | 10.1534/g3.118.200727 |
| PII | g3.118.200727 |
| PMID | 30297383 |
| PMC | PMC6288832 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Gene Expression Regulation / physiology* Gene Knockdown Techniques Oxidative Stress / physiology* Reactive Oxygen Species / metabolism* Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* |
| IF | 2.63 |
| 引用数 | 7 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 8.0 |
| リソース情報 | |
| 線虫 | tm2182 tm1817 |