RRC ID 5936
著者 Westerholm M, Roos S, Schnürer A.
タイトル Syntrophaceticus schinkii gen. nov., sp. nov., an anaerobic, syntrophic acetate-oxidizing bacterium isolated from a mesophilic anaerobic filter.
ジャーナル FEMS Microbiol Lett
Abstract A mesophilic, syntrophic acetate-oxidizing bacterium, designated strain Sp3(T), was isolated from sludge from a mesophilic methanogenic digestor operating at a high ammonium concentration (6.4 g L(-1) NH(4)(+)-N). The strain showed acetate-oxidizing ability in cocultivation with a hydrogen-consuming methanogen. Comparative 16S rRNA gene sequence analysis confirmed that strain Sp3(T) belonged to the Firmicutes-Clostridia class. The most closely related species was Thermacetogenium phaeum (16S rRNA gene sequence identity 92%). Strain Sp3(T) used ethanol, betaine and lactate as carbon and electron sources and showed growth between 25 and 40 degrees C and pH 6.0 and 8.0. Based on the phylogenetic position and the physiological characteristics of strain Sp3(T), this new syntrophic, acetate-oxidizing bacterium is proposed as the new genus and species Syntrophaceticus schinkii, with Sp3(T) (=JCM 16669(T)) as the type strain. An isolate (strain Esp=JCM 16670) with high 16S rRNA gene sequence identity (99%) to syntrophic acetate-oxidizing Clostridium ultunense was also retrieved from the methanogenic digestor.
巻・号 309(1)
ページ 100-4
公開日 2010-8-1
DOI 10.1111/j.1574-6968.2010.02023.x
PII FML2023
PMID 20546311
MeSH Acetates / metabolism* Anaerobiosis Bioreactors / microbiology DNA, Bacterial / genetics DNA, Ribosomal / genetics Gram-Positive Bacteria / classification Gram-Positive Bacteria / genetics Gram-Positive Bacteria / isolation & purification* Gram-Positive Bacteria / metabolism* Molecular Sequence Data Oxidation-Reduction Phylogeny RNA, Ribosomal, 16S / genetics Sewage / microbiology
IF 1.987
引用数 131
WOS 分野 MICROBIOLOGY
リソース情報
一般微生物 JCM 16669 JCM 16670