RRC ID |
5936
|
著者 |
Westerholm M, Roos S, Schnürer A.
|
タイトル |
Syntrophaceticus schinkii gen. nov., sp. nov., an anaerobic, syntrophic acetate-oxidizing bacterium isolated from a mesophilic anaerobic filter.
|
ジャーナル |
FEMS Microbiol Lett
|
Abstract |
A mesophilic, syntrophic acetate-oxidizing bacterium, designated strain Sp3(T), was isolated from sludge from a mesophilic methanogenic digestor operating at a high ammonium concentration (6.4 g L(-1) NH(4)(+)-N). The strain showed acetate-oxidizing ability in cocultivation with a hydrogen-consuming methanogen. Comparative 16S rRNA gene sequence analysis confirmed that strain Sp3(T) belonged to the Firmicutes-Clostridia class. The most closely related species was Thermacetogenium phaeum (16S rRNA gene sequence identity 92%). Strain Sp3(T) used ethanol, betaine and lactate as carbon and electron sources and showed growth between 25 and 40 degrees C and pH 6.0 and 8.0. Based on the phylogenetic position and the physiological characteristics of strain Sp3(T), this new syntrophic, acetate-oxidizing bacterium is proposed as the new genus and species Syntrophaceticus schinkii, with Sp3(T) (=JCM 16669(T)) as the type strain. An isolate (strain Esp=JCM 16670) with high 16S rRNA gene sequence identity (99%) to syntrophic acetate-oxidizing Clostridium ultunense was also retrieved from the methanogenic digestor.
|
巻・号 |
309(1)
|
ページ |
100-4
|
公開日 |
2010-8-1
|
DOI |
10.1111/j.1574-6968.2010.02023.x
|
PII |
FML2023
|
PMID |
20546311
|
MeSH |
Acetates / metabolism*
Anaerobiosis
Bioreactors / microbiology
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Gram-Positive Bacteria / classification
Gram-Positive Bacteria / genetics
Gram-Positive Bacteria / isolation & purification*
Gram-Positive Bacteria / metabolism*
Molecular Sequence Data
Oxidation-Reduction
Phylogeny
RNA, Ribosomal, 16S / genetics
Sewage / microbiology
|
IF |
1.987
|
引用数 |
131
|
WOS 分野
|
MICROBIOLOGY
|
リソース情報 |
一般微生物 |
JCM 16669
JCM 16670 |