Reference - Detail
| RRC ID | 59438 |
|---|---|
| Author | Andrusiak MG, Sharifnia P, Lyu X, Wang Z, Dickey AM, Wu Z, Chisholm AD, Jin Y. |
| Title | Inhibition of Axon Regeneration by Liquid-like TIAR-2 Granules. |
| Journal | Neuron |
| Abstract |
Phase separation into liquid-like compartments is an emerging property of proteins containing prion-like domains (PrLDs), yet the in vivo roles of phase separation remain poorly understood. TIA proteins contain a C-terminal PrLD, and mutations in the PrLD are associated with several diseases. Here, we show that the C. elegans TIAR-2/TIA protein functions cell autonomously to inhibit axon regeneration. TIAR-2 undergoes liquid-liquid phase separation in vitro and forms granules with liquid-like properties in vivo. Axon injury induces a transient increase in TIAR-2 granule number. The PrLD is necessary and sufficient for granule formation and inhibiting regeneration. Tyrosine residues within the PrLD are important for granule formation and inhibition of regeneration. TIAR-2 is also serine phosphorylated in vivo. Non-phosphorylatable TIAR-2 variants do not form granules and are unable to inhibit axon regeneration. Our data demonstrate an in vivo function for phase-separated TIAR-2 and identify features critical for its function in axon regeneration. |
| Volume | 104(2) |
| Pages | 290-304.e8 |
| Published | 2019-10-23 |
| DOI | 10.1016/j.neuron.2019.07.004 |
| PII | S0896-6273(19)30602-6 |
| PMID | 31378567 |
| PMC | PMC6813885 |
| MeSH | Animals Axons / metabolism* Axons / physiology Caenorhabditis elegans Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Cell Compartmentation Cytoplasmic Granules Nerve Regeneration / physiology* Protein Domains RNA Recognition Motif Proteins / genetics RNA Recognition Motif Proteins / metabolism* RNA-Binding Proteins / genetics RNA-Binding Proteins / metabolism T-Cell Intracellular Antigen-1 / genetics T-Cell Intracellular Antigen-1 / metabolism |
| IF | 14.403 |
| Times Cited | 6 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 34 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm2923 tm361 tm2262 |