RRC ID 59462
Author Cebul ER, McLachlan IG, Heiman MG.
Title Dendrites with specialized glial attachments develop by retrograde extension using SAX-7 and GRDN-1.
Journal Development
Abstract Dendrites develop elaborate morphologies in concert with surrounding glia, but the molecules that coordinate dendrite and glial morphogenesis are mostly unknown. C. elegans offers a powerful model for identifying such factors. Previous work in this system examined dendrites and glia that develop within epithelia, similar to mammalian sense organs. Here, we focus on the neurons BAG and URX, which are not part of an epithelium but instead form membranous attachments to a single glial cell at the nose, reminiscent of dendrite-glia contacts in the mammalian brain. We show that these dendrites develop by retrograde extension, in which the nascent dendrite endings anchor to the presumptive nose and then extend by stretching during embryo elongation. Using forward genetic screens, we find that dendrite development requires the adhesion protein SAX-7/L1CAM and the cytoplasmic protein GRDN-1/CCDC88C to anchor dendrite endings at the nose. SAX-7 acts in neurons and glia, while GRDN-1 acts in glia to non-autonomously promote dendrite extension. Thus, this work shows how glial factors can help to shape dendrites, and identifies a novel molecular mechanism for dendrite growth by retrograde extension.
Volume 147(4)
Published 2020-2-17
DOI 10.1242/dev.180448
PII dev.180448
PMID 31988188
PMC PMC7044450
MeSH Alleles Animals Brain / physiology* Caenorhabditis elegans / physiology Caenorhabditis elegans Proteins / physiology* Cell Membrane / physiology Cytoplasm / physiology Dendrites / physiology Epithelium / physiology Microfilament Proteins / physiology* Neural Cell Adhesion Molecules / physiology* Neurogenesis Neuroglia / physiology* Protein Isoforms Sensory Receptor Cells / physiology
IF 5.763
Times Cited 1
C.elegans tm1869