| RRC ID |
59512
|
| Author |
Huggins HP, Subash JS, Stoffel H, Henderson MA, Hoffman JL, Buckner DS, Sengupta MS, Boag PR, Lee MH, Keiper BD.
|
| Title |
Distinct roles of two eIF4E isoforms in the germline of Caenorhabditis elegans.
|
| Journal |
J Cell Sci
|
| Abstract |
Germ cells use both positive and negative mRNA translational control to regulate gene expression that drives their differentiation into gametes. mRNA translational control is mediated by RNA-binding proteins, miRNAs and translation initiation factors. We have uncovered the discrete roles of two translation initiation factor eIF4E isoforms (IFE-1, IFE-3) that bind 7-methylguanosine (m7G) mRNA caps during Caenorhabditiselegans germline development. IFE-3 plays important roles in germline sex determination (GSD), where it promotes oocyte cell fate and is dispensable for spermatogenesis. IFE-3 is expressed throughout the germline and localizes to germ granules, but is distinct from IFE-1 and PGL-1, and facilitates oocyte growth and viability. This contrasts with the robust expression in spermatocytes of IFE-1, the isoform that resides within P granules in spermatocytes and oocytes, and promotes late spermatogenesis. Each eIF4E is localized by its cognate eIF4E-binding protein (IFE-1:PGL-1 and IFE-3:IFET-1). IFE-3 and IFET-1 regulate translation of several GSD mRNAs, but not those under control of IFE-1. Distinct mutant phenotypes, in vivo localization and differential mRNA translation suggest independent dormant and active periods for each eIF4E isoform in the germline.
|
| Volume |
133(6)
|
| Published |
2020-3-30
|
| DOI |
10.1242/jcs.237990
|
| PII |
jcs.237990
|
| PMID |
32079657
|
| PMC |
PMC7132772
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Eukaryotic Initiation Factor-4E / genetics
Male
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA, Messenger
RNA-Binding Proteins
|
| IF |
4.517
|
| Times Cited |
2
|
| Resource |
| C.elegans |
|
