RRC ID 59529
Author Lee CS, Putnam A, Lu T, He S, Ouyang JPT, Seydoux G.
Title Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins.
Journal Elife
Abstract RNA granules are protein/RNA condensates. How specific mRNAs are recruited to cytoplasmic RNA granules is not known. Here, we characterize the transcriptome and assembly of P granules, RNA granules in the C. elegans germ plasm. We find that P granules recruit mRNAs by condensation with the disordered protein MEG-3. MEG-3 traps mRNAs into non-dynamic condensates in vitro and binds to ~500 mRNAs in vivo in a sequence-independent manner that favors embryonic mRNAs with low ribosome coverage. Translational stress causes additional mRNAs to localize to P granules and translational activation correlates with P granule exit for two mRNAs coding for germ cell fate regulators. Localization to P granules is not required for translational repression but is required to enrich mRNAs in the germ lineage for robust germline development. Our observations reveal similarities between P granules and stress granules and identify intrinsically-disordered proteins as drivers of RNA condensation during P granule assembly.
Volume 9
Published 2020-1-24
DOI 10.7554/eLife.52896
PII 52896
PMID 31975687
PMC PMC7007223
MeSH Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* Cytoplasmic Granules / metabolism* Germ Cells Immunoprecipitation Intrinsically Disordered Proteins / metabolism* Protein Binding Protein Biosynthesis RNA, Messenger / metabolism*
IF 7.551
Times Cited 4
C.elegans tm4259