論文 - 詳細
| RRC ID | 59529 |
|---|---|
| 著者 | Lee CS, Putnam A, Lu T, He S, Ouyang JPT, Seydoux G. |
| タイトル | Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins. |
| ジャーナル | Elife |
| Abstract |
RNA granules are protein/RNA condensates. How specific mRNAs are recruited to cytoplasmic RNA granules is not known. Here, we characterize the transcriptome and assembly of P granules, RNA granules in the C. elegans germ plasm. We find that P granules recruit mRNAs by condensation with the disordered protein MEG-3. MEG-3 traps mRNAs into non-dynamic condensates in vitro and binds to ~500 mRNAs in vivo in a sequence-independent manner that favors embryonic mRNAs with low ribosome coverage. Translational stress causes additional mRNAs to localize to P granules and translational activation correlates with P granule exit for two mRNAs coding for germ cell fate regulators. Localization to P granules is not required for translational repression but is required to enrich mRNAs in the germ lineage for robust germline development. Our observations reveal similarities between P granules and stress granules and identify intrinsically-disordered proteins as drivers of RNA condensation during P granule assembly. |
| 巻・号 | 9 |
| 公開日 | 2020-1-24 |
| DOI | 10.7554/eLife.52896 |
| PII | 52896 |
| PMID | 31975687 |
| PMC | PMC7007223 |
| MeSH | Animals Caenorhabditis elegans / metabolism Caenorhabditis elegans Proteins / metabolism* Cytoplasmic Granules / metabolism* Germ Cells Immunoprecipitation Intrinsically Disordered Proteins / metabolism* Protein Binding Protein Biosynthesis RNA, Messenger / metabolism* |
| IF | 7.551 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 34 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm4259 |