| RRC ID |
59529
|
| 著者 |
Lee CS, Putnam A, Lu T, He S, Ouyang JPT, Seydoux G.
|
| タイトル |
Recruitment of mRNAs to P granules by condensation with intrinsically-disordered proteins.
|
| ジャーナル |
Elife
|
| Abstract |
RNA granules are protein/RNA condensates. How specific mRNAs are recruited to cytoplasmic RNA granules is not known. Here, we characterize the transcriptome and assembly of P granules, RNA granules in the C. elegans germ plasm. We find that P granules recruit mRNAs by condensation with the disordered protein MEG-3. MEG-3 traps mRNAs into non-dynamic condensates in vitro and binds to ~500 mRNAs in vivo in a sequence-independent manner that favors embryonic mRNAs with low ribosome coverage. Translational stress causes additional mRNAs to localize to P granules and translational activation correlates with P granule exit for two mRNAs coding for germ cell fate regulators. Localization to P granules is not required for translational repression but is required to enrich mRNAs in the germ lineage for robust germline development. Our observations reveal similarities between P granules and stress granules and identify intrinsically-disordered proteins as drivers of RNA condensation during P granule assembly.
|
| 巻・号 |
9
|
| 公開日 |
2020-1-24
|
| DOI |
10.7554/eLife.52896
|
| PII |
52896
|
| PMID |
31975687
|
| PMC |
PMC7007223
|
| MeSH |
Animals
Caenorhabditis elegans / metabolism
Caenorhabditis elegans Proteins / metabolism*
Cytoplasmic Granules / metabolism*
Germ Cells
Immunoprecipitation
Intrinsically Disordered Proteins / metabolism*
Protein Binding
Protein Biosynthesis
RNA, Messenger / metabolism*
|
| IF |
7.551
|
| 引用数 |
4
|
| リソース情報 |
| 線虫 |
tm4259 |
