| RRC ID |
59535
|
| 著者 |
Lev I, Toker IA, Mor Y, Nitzan A, Weintraub G, Antonova O, Bhonkar O, Ben Shushan I, Seroussi U, Claycomb JM, Anava S, Gingold H, Zaidel-Bar R, Rechavi O.
|
| タイトル |
Germ Granules Govern Small RNA Inheritance.
|
| ジャーナル |
Curr Biol
|
| Abstract |
In C. elegans nematodes, components of liquid-like germ granules were shown to be required for transgenerational small RNA inheritance. Surprisingly, we show here that mutants with defective germ granules can nevertheless inherit potent small RNA-based silencing responses, but some of the mutants lose this ability after many generations of homozygosity. Animals mutated in pptr-1, which is required for stabilization of P granules in the early embryo, display extraordinarily strong heritable RNAi responses, lasting for tens of generations. Intriguingly, the RNAi capacity of descendants derived from mutants defective in the core germ granule proteins MEG-3 and MEG-4 is determined by the genotype of the ancestors and changes transgenerationally. Further, whether the meg-3/4 mutant alleles were present in the paternal or maternal lineages leads to different transgenerational consequences. Small RNA inheritance, rather than maternal contribution of the germ granules themselves, mediates the transgenerational defects in RNAi of meg-3/4 mutants and their progeny. Accordingly, germ granule defects lead to heritable genome-wide mis-expression of endogenous small RNAs. Upon disruption of germ granules, hrde-1 mutants can inherit RNAi, although HRDE-1 was previously thought to be absolutely required for RNAi inheritance. We propose that germ granules sort and shape the RNA pool, and that small RNA inheritance maintains this activity for multiple generations.
|
| 巻・号 |
29(17)
|
| ページ |
2880-2891.e4
|
| 公開日 |
2019-9-9
|
| DOI |
10.1016/j.cub.2019.07.054
|
| PII |
S0960-9822(19)30934-0
|
| PMID |
31378614
|
| PMC |
PMC6739422
|
| MeSH |
Animals
Caenorhabditis elegans / genetics*
Germ Cells / metabolism*
Inheritance Patterns*
RNA, Helminth / genetics*
RNA, Small Interfering / genetics*
|
| IF |
9.193
|
| 引用数 |
5
|
| リソース情報 |
| 線虫 |
tm3103
tm4259
tm1019
tm1200 |
