| RRC ID |
59607
|
| 著者 |
Takagaki N, Ohta A, Ohnishi K, Kawanabe A, Minakuchi Y, Toyoda A, Fujiwara Y, Kuhara A.
|
| タイトル |
The mechanoreceptor DEG-1 regulates cold tolerance in Caenorhabditis elegans.
|
| ジャーナル |
EMBO Rep
|
| Abstract |
Caenorhabditis elegans mechanoreceptors located in ASG sensory neurons have been found to sense ambient temperature, which is a key trait for animal survival. Here, we show that experimental loss of xanthine dehydrogenase (XDH-1) function in AIN and AVJ interneurons results in reduced cold tolerance and atypical neuronal response to changes in temperature. These interneurons connect with upstream neurons such as the mechanoreceptor-expressing ASG. Ca2+ imaging revealed that ASG neurons respond to warm temperature via the mechanoreceptor DEG-1, a degenerin/epithelial Na+ channel (DEG/ENaC), which in turn affects downstream AIN and AVJ circuits. Ectopic expression of DEG-1 in the ASE gustatory neuron results in the acquisition of warm sensitivity, while electrophysiological analysis revealed that DEG-1 and human MDEG1 were involved in warm sensation. Taken together, these results suggest that cold tolerance is regulated by mechanoreceptor-mediated circuit calculation.
|
| 巻・号 |
21(3)
|
| ページ |
e48671
|
| 公開日 |
2020-3-4
|
| DOI |
10.15252/embr.201948671
|
| PMID |
32009302
|
| PMC |
PMC7054665
|
| MeSH |
Animals
Caenorhabditis elegans* / genetics
Caenorhabditis elegans* / metabolism
Caenorhabditis elegans Proteins* / genetics
Caenorhabditis elegans Proteins* / metabolism
Cold Temperature*
Humans
Mechanoreceptors / metabolism
Membrane Proteins
Sensory Receptor Cells / metabolism
Sodium Channels
|
| IF |
8.383
|
| 引用数 |
1
|
| リソース情報 |
| 線虫 |
tm7280
tm10725
tm5192
tm2994
tm1163
tm3455
tm4022
tm7132
tm3007
tm1987
tm6528
tm776
tm3294 |
