論文 - 詳細
| RRC ID | 59632 |
|---|---|
| 著者 | Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S. |
| タイトル | Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation. |
| ジャーナル | Cell Rep |
| Abstract |
piRNAs play significant roles in suppressing transposons and nonself nucleic acids, maintaining genome integrity, and defending against viral infections. In C. elegans, piRNA precursors are transcribed in the nucleus and are subjected to a number of processing and maturation steps. The biogenesis of piRNAs is not fully understood. We use functional proteomics in C. elegans and identify a piRNA biogenesis and chromosome segregation (PICS) complex. The PICS complex contains TOFU-6, PID-1, PICS-1, TOST-1, and ERH-2, which exhibit dynamic localization among different subcellular compartments. In the germlines, the PICS complex contains TOFU-6/PICS-1/ERH-2/PID-1, is largely concentrated at the perinuclear granule zone, and engages in piRNA processing. During embryogenesis, the TOFU-6/PICS-1/ERH-2/TOST-1 complex accumulates in the nucleus and plays essential roles in chromosome segregation. The functions of these factors in mediating chromosome segregation are independent of piRNA production. We speculate that differential compositions of PICS factors may help cells coordinate distinct cellular processes. |
| 巻・号 | 27(12) |
| ページ | 3561-3572.e3 |
| 公開日 | 2019-6-18 |
| DOI | 10.1016/j.celrep.2019.05.076 |
| PII | S2211-1247(19)30705-3 |
| PMID | 31216475 |
| MeSH | Animals Caenorhabditis elegans / genetics Caenorhabditis elegans / growth & development Caenorhabditis elegans / metabolism* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Chromosome Segregation* Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Gene Expression Regulation* Germ Cells / cytology Germ Cells / metabolism Proteome / analysis* RNA, Small Interfering / biosynthesis* RNA, Small Interfering / genetics* |
| IF | 7.815 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm6709 tm6157 tm869 tm6424 tm2417 |