RRC ID 59661
Author Machino R, Matsumoto K, Taniguchi D, Tsuchiya T, Takeoka Y, Taura Y, Moriyama M, Tetsuo T, Oyama S, Takagi K, Miyazaki T, Hatachi G, Doi R, Shimoyama K, Matsuo N, Yamasaki N, Nakayama K, Nagayasu T.
Title Replacement of Rat Tracheas by Layered, Trachea-Like, Scaffold-Free Structures of Human Cells Using a Bio-3D Printing System.
Journal Adv Healthc Mater
Abstract Current scaffold-based tissue engineering approaches are subject to several limitations, such as design inflexibility, poor cytocompatibility, toxicity, and post-transplant degradation. Thus, scaffold-free tissue-engineered structures can be a promising solution to overcome the issues associated with classical scaffold-based materials in clinical transplantation. The present study seeks to optimize the culture conditions and cell combinations used to generate scaffold-free structures using a Bio-3D printing system. Human cartilage cells, human fibroblasts, human umbilical vein endothelial cells, and human mesenchymal stem cells from bone marrow are aggregated into spheroids and placed into a Bio-3D printing system with dedicated needles positioned according to 3D configuration data, to develop scaffold-free trachea-like tubes. Culturing the Bio-3D-printed structures with proper flow of specific medium in a bioreactor facilitates the rearrangement and self-organization of cells, improving physical strength and tissue function. The Bio-3D-printed tissue forms small-diameter trachea-like tubes that are implanted into rats with the support of catheters. It is confirmed that the tubes are viable in vivo and that the tracheal epithelium and capillaries proliferate. This tissue-engineered, scaffold-free, tubular structure can represent a significant step toward clinical application of bioengineered organs.
Volume 8(7)
Pages e1800983
Published 2019-4-1
DOI 10.1002/adhm.201800983
PMID 30632706
MeSH Animals Bioprinting / methods* Cell Differentiation Chondrocytes / cytology Chondrocytes / metabolism Glycosaminoglycans / chemistry Humans Mesenchymal Stem Cells / cytology Printing, Three-Dimensional* Rats Spheroids, Cellular / cytology Spheroids, Cellular / metabolism Spheroids, Cellular / transplantation Tensile Strength Tissue Engineering Tissue Scaffolds / chemistry Trachea / chemistry* Trachea / pathology
IF 6.27
Times Cited 8
Resource
Human and Animal Cells MC3T3-E1(RCB1126)