RRC ID 59665
著者 Hontani K, Onodera T, Terashima M, Momma D, Matsuoka M, Baba R, Joutoku Z, Matsubara S, Homan K, Hishimura R, Xu L, Iwasaki N.
タイトル Chondrogenic differentiation of mouse induced pluripotent stem cells using the three-dimensional culture with ultra-purified alginate gel.
ジャーナル J Biomed Mater Res A
Abstract As articular cartilages have rarely healed by themselves because of their characteristics of avascularity and low cell density, surgical intervention is ideal for patients with cartilaginous injuries. Because of structural characteristics of the cartilage tissue, a three-dimensional culture of stem cells in biomaterials is a favorable system on cartilage tissue engineering. Induced pluripotent stem cells (iPSCs) are a new cell source in cartilage tissue engineering for its characteristics of self-renewal capability and pluripotency. However, the optimal cultivation condition for chondrogenesis of iPSCs is still unknown. Here we show that a novel chondrogenic differentiation method of iPSCs using the combination of three-dimensional cultivation in ultra-purified alginate gel (UPAL gel) and multi-step differentiation via mesenchymal stem cell-like cells (iPS-MSCs) could efficiently and specifically differentiate iPSCs into chondrocytes. The iPS-MSCs in UPAL gel culture sequentially enhanced the expression of chondrogenic marker without the upregulation of that of osteogenic and adipogenic marker and histologically showed homogeneous chondrogenic extracellular matrix formation. Our results suggest that the pluripotency of iPSCs can be controlled when iPSCs are differentiated into iPS-MSCs before embedding in UPAL gel. These results lead to the establishment of an efficient three-dimensional system to engineer artificial cartilage tissue from iPSCs for cartilage regeneration. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1086-1093, 2019.
巻・号 107(5)
ページ 1086-1093
公開日 2019-5-1
DOI 10.1002/jbm.a.36615
PMID 30665260
MeSH Alginates / pharmacology* Animals Biomarkers / metabolism Cell Culture Techniques / methods* Cell Differentiation* / drug effects Cell Shape / drug effects Chondrogenesis* / drug effects Gels / pharmacology* Gene Expression Regulation / drug effects Induced Pluripotent Stem Cells / cytology* Mesenchymal Stem Cells / cytology Mesenchymal Stem Cells / drug effects Mesoderm / metabolism Mice, Inbred BALB C
IF 3.221
引用数 0
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)