Reference - Detail
| RRC ID | 5966 |
|---|---|
| Author | Kimura T, Naruse K. |
| Title | M-marker 2009, a marker set for mapping medaka mutants using PCR length polymorphisms with an automated microchip gel electrophoresis system. |
| Journal | Biotechniques |
| Abstract |
We have developed a highly effective marker set for assigning medaka mutants to specific linkage groups (LG) in bulk segregation analysis by PCR product length polymorphisms (PLPs). The marker set permits analysis using an automated microchip electrophoresis system as well as conventional agarose gel electrophoresis. |
| Volume | 49(2) |
| Pages | 582-3 |
| Published | 2010-8-1 |
| DOI | 10.2144/000113467 |
| PII | 000113467 |
| PMID | 20701594 |
| MeSH | Animals Automation Chromosome Mapping / methods* Chromosome Segregation / genetics Electrophoresis, Agar Gel / methods* Electrophoresis, Microchip / methods* Genetic Markers Mutation / genetics* Oryzias / genetics* Polymerase Chain Reaction / methods* Polymorphism, Restriction Fragment Length / genetics* |
| IF | 1.541 |
| Times Cited | 10 |
| WOS Category | BIOCHEMICAL RESEARCH METHODS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| Resource | |
| Rats | |
| Medaka | IB186 (HNI-II) IB833 (Kaga) IB178 (HdrR-II1) IB183 (HSOK) MT76 (lf) MT89 (pl-2) |