RRC ID 5968
Author Nakamura S, Kobayashi K, Nishimura T, Higashijima S, Tanaka M.
Title Identification of germline stem cells in the ovary of the teleost medaka.
Journal Science
Abstract Germline stem cells continually produce sperm in vertebrate testes, whereas there is no direct evidence showing that germline stem cells are present in adult vertebrate ovaries. By using transgenic methods and clonal analysis, we identified germline stem cells that supported oogenesis and the production of offspring in the ovaries of adult medaka fish. Early-stage germ cells were localized in clusters along interwoven threadlike cords of sox9b-expressing somatic cells (termed germinal cradles) where the germ cells developed. Germline stem cells gave rise to germ cells that divided to produce cysts, which then underwent cell death or separated to form follicles. Our results provide insight into the germline stem cell biology of medaka and provide a model system for studying vertebrate stem cell niches.
Volume 328(5985)
Pages 1561-3
Published 2010-6-18
DOI 10.1126/science.1185473
PII science.1185473
PMID 20488987
MeSH Animals Animals, Genetically Modified Cell Cycle Cell Movement Female Germ Cells / cytology* Germ Cells / physiology Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Hot Temperature Meiosis Mitosis Oocytes / cytology* Oocytes / physiology Oogenesis* Oogonia / cytology Oogonia / physiology Oryzias* / genetics Oryzias* / physiology Ovarian Follicle / cytology Ovary / cytology* SOX9 Transcription Factor / genetics SOX9 Transcription Factor / metabolism Stem Cell Niche* Stem Cells / cytology* Stem Cells / physiology
IF 41.846
Times Cited 140
Medaka OK-Cab (MT830)