Reference - Detail
| RRC ID | 59701 |
|---|---|
| Author | Hibino M, Yamada Y, Fujishita N, Sato Y, Maeki M, Tokeshi M, Harashima H. |
| Title | The Use of a Microfluidic Device to Encapsulate a Poorly Water-Soluble Drug CoQ10 in Lipid Nanoparticles and an Attempt to Regulate Intracellular Trafficking to Reach Mitochondria. |
| Journal | J Pharm Sci |
| Abstract |
A number of drugs that are currently on the market, as well as new candidates for drugs, are poorly water soluble. Because of this, a need exists to develop drug formulations that will permit the expanded use of such drugs. The use of liposomes and lipid nanoparticles for drug delivery has attracted attention as a technique for solubilizing molecules that are poorly water soluble, but this technique faces serious scale-up risks. In this study, we report on attempts to encapsulate Coenzyme Q10 (CoQ10) as a model of a poorly water-soluble drug in an MITO-Porter, a liposome for mitochondrial delivery using a microfluidic device (a CoQ10-MITO-Porter [μ]). The physical properties of the CoQ10-MITO-Porter [μ] including homogeneity, size, and preparation volume were compared with those for a CoQ10-MITO-Porter prepared by the ethanol dilution method (a CoQ10-MITO-Porter [ED]). In the case where a microfluidic device was used, a small-sized CoQ10-MITO-Porter was formed homogeneously, and it was possible to prepare it on a large scale. Intracellular observations using HeLa cells showed that the CoQ10-MITO-Porter [μ] was efficiently internalized by cells to reach mitochondria. These results indicate that the CoQ10-MITO-Porter [μ] represents a potential candidate for use in mitochondrial nanomedicine. |
| Volume | 108(8) |
| Pages | 2668-2676 |
| Published | 2019-8-1 |
| DOI | 10.1016/j.xphs.2019.04.001 |
| PII | S0022-3549(19)30214-X |
| PMID | 30959057 |
| MeSH | HeLa Cells Humans Lab-On-A-Chip Devices Lipid Metabolism Lipids / chemistry Liposomes / chemistry Liposomes / metabolism* Mitochondria / metabolism* Solubility Ubiquinone / administration & dosage Ubiquinone / analogs & derivatives* Ubiquinone / chemistry Ubiquinone / pharmacokinetics Vitamins / administration & dosage* Vitamins / chemistry Vitamins / pharmacokinetics Water / chemistry |
| IF | 3.197 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa |