RRC ID 59731
Author Imaeda A, Tomoike F, Hayakawa M, Nakamoto K, Kimura Y, Abe N, Abe H.
Title N6-methyl adenosine in siRNA evades immune response without reducing RNAi activity.
Journal Nucleosides Nucleotides Nucleic Acids
Abstract siRNA is a powerful method to suppress specific gene expression and has recently been utilized for molecular biology as well as medicine. However, introduction of dsRNA stimulates immune-responses as side-effects. In the present study, we utilized N6-methyl adenosine, one of the natural modified nucleosides, instead of adenosine in siRNA. When adenosine in the passenger or guide strand of siRNA was completely replaced with N6-methyl adenosine, the immune response against siRNA was evaded without any reduction in RNAi activity. This knowledge will promote the medical application of siRNA and enhance our understanding on cellular discrimination of non-self and self dsRNA.
Volume 38(12)
Pages 972-979
Published 2019
DOI 10.1080/15257770.2019.1641205
PMID 31298608
MeSH Adenosine / analogs & derivatives* Adenosine / chemistry Base Sequence Gene Expression HeLa Cells Humans RNA Interference / immunology* RNA, Double-Stranded / chemistry RNA, Double-Stranded / metabolism RNA, Small Interfering / chemistry* RNA, Small Interfering / metabolism*
IF 1.167
Times Cited 3
Human and Animal Cells T98G(RCB1954)