論文 - 詳細
| RRC ID | 59741 |
|---|---|
| 著者 | Yamada S, Kanda Y. |
| タイトル | Retinoic acid promotes barrier functions in human iPSC-derived intestinal epithelial monolayers. |
| ジャーナル | J Pharmacol Sci |
| Abstract |
Vitamin A (VA) is a fat-soluble micronutrient that plays essential roles in various biological processes, including cell growth, differentiation, and apoptosis. In the intestine, VA are known to promote mucosal homeostasis and immunity. However, the effect of VA in intestinal development has not been well elucidated. In the present study, we generated human intestine organoids from human induced pluripotent stem cells (iPSCs), and investigated the effect of the VA active metabolite all-trans retinoic acid (RA), on differentiation into intestinal organoids. As a result, RA increased the gene expression of a drug-metabolizing enzyme CYP3A4, as a functional molecule of intestinal mature development, in iPSC-derived intestinal organoids. In addition, RA increased transepithelial electrical resistance, an indicator of epithelial integrity, and decreased the permeability of monolayers to fluorescein isothiocyanate-labeled dextran in intestinal epithelial monolayers. Finally, RA increased the expression of ZO-1, a marker of tight junctions, which are essential for intestinal epithelial barrier function. Taken together, these results indicate that RA promotes barrier functions of iPSC-derived intestinal epithelial monolayers by increasing ZO-1 expression. |
| 巻・号 | 140(4) |
| ページ | 337-344 |
| 公開日 | 2019-8-1 |
| DOI | 10.1016/j.jphs.2019.06.012 |
| PII | S1347-8613(19)35687-7 |
| PMID | 31399314 |
| MeSH | Apoptosis / drug effects Caco-2 Cells Cell Differentiation / drug effects Epithelial Cells / drug effects* Epithelial Cells / metabolism Humans Induced Pluripotent Stem Cells / drug effects* Induced Pluripotent Stem Cells / metabolism Intestinal Mucosa / drug effects Intestinal Mucosa / metabolism Permeability / drug effects Tight Junctions / drug effects Tight Junctions / metabolism Tretinoin / pharmacology* Vitamin A / pharmacology Zonula Occludens-1 Protein / metabolism |
| IF | 2.835 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 253G1(HPS0002) |