RRC ID 59762
Author Nishizuka M, Komada R, Imagawa M.
Title Knockdown of RhoE Expression Enhances TGF-β-Induced EMT (epithelial-to-mesenchymal transition) in Cervical Cancer HeLa Cells.
Journal Int J Mol Sci
Abstract Cervical cancer with early metastasis of the primary tumor is associated with poor prognosis and poor therapeutic outcomes. Since epithelial-to-mesenchymal transition (EMT) plays a role in acquisition of the ability to invade the pelvic lymph nodes and surrounding tissue, it is important to clarify the molecular mechanism underlying EMT in cervical cancer. RhoE, also known as Rnd3, is a member of the Rnd subfamily of Rho GTPases. While previous reports have suggested that RhoE may act as either a positive or a negative regulator of cancer metastasis and EMT, the role of RhoE during EMT in cervical cancer cells remains unclear. The present study revealed that RhoE expression was upregulated during transforming growth factor-β (TGF-β)-mediated EMT in human cervical cancer HeLa cells. Furthermore, reduced RhoE expression enhanced TGF-β-mediated EMT and migration of HeLa cells. In addition, we demonstrated that RhoE knockdown elevated RhoA activity and a ROCK inhibitor partially suppressed the acceleration of TGF-β-mediated EMT by RhoE knockdown. These results indicate that RhoE suppresses TGF-β-mediated EMT, partially via RhoA/ROCK signaling in cervical cancer HeLa cells.
Volume 20(19)
Published 2019-9-22
DOI 10.3390/ijms20194697
PII ijms20194697
PMID 31546735
PMC PMC6801947
MeSH Cell Line, Tumor Cell Proliferation / drug effects Epithelial-Mesenchymal Transition / drug effects Epithelial-Mesenchymal Transition / genetics* Gene Expression Regulation Gene Knockdown Techniques HeLa Cells Humans Transforming Growth Factor beta / metabolism* Transforming Growth Factor beta / pharmacology rho GTP-Binding Proteins / genetics*
IF 4.183
Times Cited 2
Human and Animal Cells HeLa