| RRC ID |
5978
|
| 著者 |
Adachi H, Shimizu K, Hattori H, Tanaka R, Chibana H, Takagi Y, Tomita Y, Kanbe T.
|
| タイトル |
Genotyping of Candida albicans by fragment analysis of microsatellites combined with 25S rDNA and RPS-based strategies.
|
| ジャーナル |
Nihon Ishinkin Gakkai Zasshi
|
| Abstract |
Because of its high discriminatory potential, fragment analysis of microsatellites has been frequently used for genotyping of Candida albicans at the strain level. In order to evaluate a genotyping system based on the fragment analysis of microsatellites combined with PCRs targeting 25S rDNA and RPS, 456 independent strains of C. albicans were subjected to genotype analysis using 4 microsatellite markers (CDC3, HIS3, CA I and CA III), followed by 25S rDNA and RPS-based genotyping. The fragment analysis using CA I showed the highest discriminatory potential (DP=0.9782), followed by HIS3 (DP=0.8780). Using combined microsatellite markers, 456 C. albicans strains were divided into 384 genotypes (DP=0.9984). PCRs targeting 25S rDNA and RPS were performed to differentiate the strains that showed identical genotypes in the fragment analysis, resulting in 434 genotypes (DP=0.9996). The combined genotyping system showed high discriminatory power at the strain level, and therefore is useful for rapid genotyping in molecular epidemiological studies of candidiasis.
|
| 巻・号 |
50(3)
|
| ページ |
167-74
|
| 公開日 |
2009-1-1
|
| DOI |
10.3314/jjmm.50.167
|
| PII |
JST.JSTAGE/jjmm/50.167
|
| PMID |
19654450
|
| MeSH |
Base Sequence
Candida albicans / genetics*
DNA, Fungal / genetics*
DNA, Ribosomal / genetics*
Genotype*
Humans
Microsatellite Repeats*
Molecular Sequence Data
RNA, Ribosomal / genetics
Random Amplified Polymorphic DNA Technique / methods*
|
| リソース情報 |
| 病原微生物 |
C. albicans? |
