RRC ID 59819
Author Takada H, Fukushima-Tanaka S, Morita M, Kasahara Y, Watanabe S, Chibazakura T, Hara H, Matsumoto K, Yoshikawa H.
Title An essential enzyme for phospholipid synthesis associates with the Bacillus subtilis divisome.
Journal Mol Microbiol
Abstract The mechanism by which the membrane synthetic machinery might be co-organized with the cell-division architecture during the bacterial cell cycle remains to be investigated. We characterized a key enzyme of phospholipid and fatty acid synthesis in Bacillus subtilis, the acyl-acyl carrier protein phosphate acyltransferase (PlsX), and identified it as a component of the cell-division machinery. Comprehensive interaction analysis revealed that PlsX interacts with FtsA, the FtsZ-anchoring protein. PlsX mainly localized at the potential division site independent of FtsA and FtsZ and then colocalized with FtsA. By multidirectional approaches, we revealed that the Z-ring stabilizes the association of PlsX at the septum and pole. The localization of PlsX is also affected by the progression of DNA replication. PlsX is needed for cell division and its inactivation leads to aberrant Z-ring formation. We propose that PlsX localization is prior to Z-ring formation in the hierarchy of septum formation events and that PlsX is important for co-ordinating membrane synthesis with cell division in order to properly complete septum formation.
Volume 91(2)
Pages 242-55
Published 2014-1-1
DOI 10.1111/mmi.12457
PMID 24224907
MeSH Bacillus subtilis / cytology Bacillus subtilis / enzymology* Bacillus subtilis / genetics Bacterial Proteins / chemistry* Bacterial Proteins / genetics Bacterial Proteins / metabolism* Cell Division Cell Polarity Cytoskeletal Proteins / metabolism* DNA Replication DNA, Bacterial / physiology Enzyme Stability
IF 3.649
Times Cited 22
Resource
Prokaryotes B. subtilis MBS699 MBS700 MBS701 MBS702 MBS703 MBS704 MBS705 MBS706 MBS707 MBS708 MBS709 MBS710 MBS711 MBS712 MBS713 MBS714 MBS715 MBS716 MBS717 MBS718 MBS719 MBS720 MBS721 MBS722 MBS723 MBS724 MBS725 MBS726 MBS727 MBS728