Reference - Detail
| RRC ID | 59828 |
|---|---|
| Author | Ikeda T, Ichikawa K, Shigeto H, Ishida T, Hirota R, Funabashi H, Kuroda A. |
| Title | Arginine-mediated dissociation of single cells and cell sheets from a polystyrene culture dish. |
| Journal | Biosci Biotechnol Biochem |
| Abstract |
Here, we report a novel non-enzymatic cell dissociation method, based on our finding that adherent cells dissociate rapidly from the polystyrene culture dish when incubated in an l- or d-arginine-containing solution. We also demonstrate the successful detachment of confluent NIH/3T3 cell monolayers from the culture dish as a cell sheet by the addition of an arginine solution. |
| Volume | 83(12) |
| Pages | 2272-2275 |
| Published | 2019-12-1 |
| DOI | 10.1080/09168451.2019.1659716 |
| PMID | 31482750 |
| MeSH | Animals Arginine / pharmacology* Cell Culture Techniques / instrumentation* Cell Survival Mice NIH 3T3 Cells Polystyrenes* Solutions |
| IF | 1.297 |
| Times Cited | 1 |
| Resource | |
| Human and Animal Cells | 293(RCB1637) NIH/3T3(RCB2767) PC-12(RCB0009) |