論文 - 詳細
| RRC ID | 59842 |
|---|---|
| 著者 | Tamai R, Kiyoura Y. |
| タイトル | Heat-killed Candida albicans augments synthetic bacterial component-induced proinflammatory cytokine production. |
| ジャーナル | Folia Microbiol (Praha) |
| Abstract |
Candida albicans can enhance the invasion of oral epithelial cells by Porphyromonas gingivalis, although the fungus is not a periodontal pathogen. In this study, we investigated whether C. albicans augments proinflammatory cytokine production by mouse macrophage-like J774.1 cells incubated with synthetic bacterial components. Mouse macrophage-like J774.1 cells, mouse primary splenocytes, human THP-1 cells, and A549 cells were pretreated with or without heat-killed C. albicans (HKCA) or substitutes for C. albicans cell wall components in 96-well flat-bottomed plates. Cells were then washed and incubated with Pam3CSK4, a Toll-like receptor (TLR) 2 ligand, or lipid A, a TLR4 ligand. Culture supernatants were analyzed by ELISA for secreted IL-6, MCP-1, TNF-α, and IL-8. HKCA augmented TLR ligand-induced proinflammatory cytokine production by J774.1 cells, mouse splenocytes, and THP-1 cells, but not A549 cells. However, IL-6, MCP-1, and TNF-α production induced by Pam3CSK4 or lipid A was not augmented when cells were pretreated with curdlan, a dectin-1 ligand, or mannan, a dectin-2 ligand. In contrast, pretreatment of cells with TLR ligands upregulated the production of IL-6 and TNF-α, but not MCP-1, induced by Pam3CSK4 or lipid A. The results suggest that C. albicans augments synthetic bacterial component-induced cytokine production by J774.1 cells via the TLR pathway, but not the dectin-1 or dectin-2 pathway. |
| 巻・号 | 64(4) |
| ページ | 555-566 |
| 公開日 | 2019-7-1 |
| DOI | 10.1007/s12223-019-00679-2 |
| PII | 10.1007/s12223-019-00679-2 |
| PMID | 30656591 |
| MeSH | Animals Bacteroidaceae Infections / genetics Bacteroidaceae Infections / immunology* Bacteroidaceae Infections / microbiology Candida albicans / chemistry Candida albicans / physiology* Cell Line Cytokines / genetics Cytokines / immunology* Hot Temperature Humans Interleukin-6 / genetics Interleukin-6 / immunology Lectins, C-Type / genetics Lectins, C-Type / immunology Macrophages / immunology Macrophages / microbiology Mice Porphyromonas gingivalis / chemistry Porphyromonas gingivalis / physiology Toll-Like Receptor 2 / genetics Toll-Like Receptor 2 / immunology Tumor Necrosis Factor-alpha / genetics Tumor Necrosis Factor-alpha / immunology |
| IF | 1.448 |
| 引用数 | 0 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | A549 |