| RRC ID |
59863
|
| Author |
Arifuzzaman M, Ito A, Ikeda K, Kawabe Y, Kamihira M.
|
| Title |
Fabricating Muscle-Neuron Constructs with Improved Contractile Force Generation.
|
| Journal |
Tissue Eng Part A
|
| Abstract |
IMPACT STATEMENT:In this study, we fabricated innervated skeletal muscle tissue constructs comprising C2C12 myoblasts and PC12 neural cells using a magnetic force-based tissue engineering technique. We found that the C2C12/PC12 co-culture enhanced neural differentiation of PC12 cells and sarcomere formation of C2C12 myotubes, accompanying with neuromuscular junction formation. The innervated skeletal muscle tissue constructs generated significantly higher contractile forces compared with aneural (C2C12 monoculture) skeletal muscle tissue constructs. These innervated skeletal muscle tissue constructs can be a useful tool for drug testing and biological research for neuromuscular diseases.
|
| Volume |
25(7-8)
|
| Pages |
563-574
|
| Published |
2019-4-1
|
| DOI |
10.1089/ten.TEA.2018.0165
|
| PMID |
30221587
|
| MeSH |
Animals
Cell Differentiation / physiology
Muscle Contraction / genetics
Muscle Contraction / physiology*
Muscle Fibers, Skeletal / cytology
Muscle Fibers, Skeletal / metabolism
Muscle, Skeletal / cytology*
Muscle, Skeletal / innervation
Muscle, Skeletal / metabolism
Neuromuscular Junction / cytology
Neuromuscular Junction / metabolism
Neurons / cytology*
Neurons / metabolism
PC12 Cells
Rats
Sarcomeres / metabolism
Tissue Engineering / methods
|
| IF |
3.508
|
| Times Cited |
2
|
| Resource |
| Human and Animal Cells |
C2C12(RCB0987) |
