論文 - 詳細
| RRC ID | 59888 |
|---|---|
| 著者 | Wu L, Sadhukhan A, Kobayashi Y, Ogo N, Tokizawa M, Agrahari RK, Ito H, Iuchi S, Kobayashi M, Asai A, Koyama H. |
| タイトル | Involvement of phosphatidylinositol metabolism in aluminum-induced malate secretion in Arabidopsis. |
| ジャーナル | J Exp Bot |
| Abstract |
To identify the upstream signaling of aluminum-induced malate secretion through aluminum-activated malate transporter 1 (AtALMT1), a pharmacological assay using inhibitors of human signal transduction pathways was performed. Early aluminum-induced transcription of AtALMT1 and other aluminum-responsive genes was significantly suppressed by phosphatidylinositol 4-kinase (PI4K) and phospholipase C (PLC) inhibitors, indicating that the PI4K-PLC metabolic pathway activates early aluminum signaling. Inhibitors of phosphatidylinositol 3-kinase (PI3K) and PI4K reduced aluminum-activated malate transport by AtALMT1, suggesting that both the PI3K and PI4K metabolic pathways regulate this process. These results were validated using T-DNA insertion mutants of PI4K and PI3K-RNAi lines. A human protein kinase inhibitor, putatively inhibiting homologous calcineurin B-like protein-interacting protein kinase and/or Ca-dependent protein kinase in Arabidopsis, suppressed late-phase aluminum-induced expression of AtALMT1, which was concomitant with the induction of an AtALMT1 repressor, WRKY46, and suppression of an AtALMT1 activator, Calmodulin-binding transcription activator 2 (CAMTA2). In addition, a human deubiquitinase inhibitor suppressed aluminum-activated malate transport, suggesting that deubiquitinases can regulate this process. We also found a reduction of aluminum-induced citrate secretion in tobacco by applying inhibitors of PI3K and PI4K. Taken together, our results indicated that phosphatidylinositol metabolism regulates organic acid secretion in plants under aluminum stress. |
| 巻・号 | 70(12) |
| ページ | 3329-3342 |
| 公開日 | 2019-6-28 |
| DOI | 10.1093/jxb/erz179 |
| PII | 5448962 |
| PMID | 30977815 |
| MeSH | Aluminum / metabolism* Arabidopsis / metabolism* Arabidopsis Proteins / genetics* Arabidopsis Proteins / metabolism Malates / metabolism* Organic Anion Transporters / genetics* Organic Anion Transporters / metabolism Phosphatidylinositols / metabolism* |
| IF | 5.36 |
| 引用数 | 1 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| シロイヌナズナ / 植物培養細胞・遺伝子 | sja05800 |