Reference - Detail
| RRC ID | 59913 |
|---|---|
| Author | Takeuchi T, Tamura M, Ishiwata K, Hamasaki M, Hamano S, Arata Y, Hatanaka T. |
| Title | Galectin-2 suppresses nematode development by binding to the invertebrate-specific galactoseβ1-4fucose glyco-epitope. |
| Journal | Glycobiology |
| Abstract |
Galactoseβ1-4Fucose (GalFuc) is a unique disaccharide found in invertebrates including nematodes. A fungal galectin CGL2 suppresses nematode development by recognizing the galactoseβ1-4fucose epitope. The Caenorhabditis elegans galectin LEC-6 recognizes it as an endogenous ligand and the Glu67 residue of LEC-6 is responsible for this interaction. We found that mammalian galectin-2 (Gal-2) also has a comparable glutamate residue, Glu52. In the present study, we investigated the potential nematode-suppressing activity of Gal-2 using C. elegans as a model and focusing on Gal-2 binding to the GalFuc epitope. Gal-2 suppressed C. elegans development whereas its E52D mutant (Glu52 substituted by Asp), galectin-1 and galectin-3 had little effect on C. elegans growth. Lectin-staining using fluorescently-labeled Gal-2 revealed that, like CGL2, it specifically binds to the C. elegans intestine. Natural C. elegans glycoconjugates were specifically bound by immobilized Gal-2. Western blotting with anti-GalFuc antibody showed that the bound glycoconjugates had the GalFuc epitope. Frontal affinity chromatography with pyridylamine-labeled C. elegans N-glycans disclosed that Gal-2 (but not its E52D mutant) recognizes the GalFuc epitope. Gal-2 also binds to the GalFuc-bearing glycoconjugates of Ascaris and the GalFuc epitope is present in the parasitic nematodes Nippostrongylus brasiliensis and Brugia pahangi. These results indicate that Gal-2 suppresses C. elegans development by binding to its GalFuc epitope. The findings also imply that Gal-2 may prevent infestations of various parasitic nematodes bearing the GalFuc epitope. |
| Volume | 29(6) |
| Pages | 504-512 |
| Published | 2019-6-1 |
| DOI | 10.1093/glycob/cwz022 |
| PII | 5381599 |
| PMID | 30874734 |
| MeSH | Animals Ascaris suum / growth & development Ascaris suum / metabolism Binding Sites Biomphalaria Caenorhabditis elegans / growth & development* Caenorhabditis elegans / metabolism Disaccharides / chemistry* Disaccharides / metabolism Epitopes / chemistry* Epitopes / metabolism Galectin 2 / chemistry Galectin 2 / metabolism* HeLa Cells Humans Mice Mice, Inbred ICR |
| IF | 4.194 |
| Times Cited | 2 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 2 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | HeLa(RCB0007) |