RRC ID 59917
Author Shimizu Y, Rai A, Okawa Y, Tomatsu H, Sato M, Kera K, Suzuki H, Saito K, Yamazaki M.
Title Metabolic diversification of nitrogen-containing metabolites by the expression of a heterologous lysine decarboxylase gene in Arabidopsis.
Journal Plant J
Abstract Lysine decarboxylase converts l-lysine to cadaverine as a branching point for the biosynthesis of plant Lys-derived alkaloids. Although cadaverine contributes towards the biosynthesis of Lys-derived alkaloids, its catabolism, including metabolic intermediates and the enzymes involved, is not known. Here, we generated transgenic Arabidopsis lines by expressing an exogenous lysine/ornithine decarboxylase gene from Lupinus angustifolius (La-L/ODC) and identified cadaverine-derived metabolites as the products of the emerged biosynthetic pathway. Through untargeted metabolic profiling, we observed the upregulation of polyamine metabolism, phenylpropanoid biosynthesis and the biosynthesis of several Lys-derived alkaloids in the transgenic lines. Moreover, we found several cadaverine-derived metabolites specifically detected in the transgenic lines compared with the non-transformed control. Among these, three specific metabolites were identified and confirmed as 5-aminopentanal, 5-aminopentanoate and δ-valerolactam. Cadaverine catabolism in a representative transgenic line (DC29) was traced by feeding stable isotope-labeled [α-15 N]- or [ε-15 N]-l-lysine. Our results show similar 15 N incorporation ratios from both isotopomers for the specific metabolite features identified, indicating that these metabolites were synthesized via the symmetric structure of cadaverine. We propose biosynthetic pathways for the metabolites on the basis of metabolite chemistry and enzymes known or identified through catalyzing specific biochemical reactions in this study. Our study shows that this pool of enzymes with promiscuous activities is the driving force for metabolite diversification in plants. Thus, this study not only provides valuable information for understanding the catabolic mechanism of cadaverine but also demonstrates that cadaverine accumulation is one of the factors to expand plant chemodiversity, which may lead to the emergence of Lys-derived alkaloid biosynthesis.
Volume 100(3)
Pages 505-521
Published 2019-11-1
DOI 10.1111/tpj.14454
PMID 31364191
PMC PMC6899585
MeSH Alkaloids / metabolism Arabidopsis / genetics Arabidopsis / metabolism* Biosynthetic Pathways Cadaverine / metabolism* Carboxy-Lyases / genetics Carboxy-Lyases / metabolism* Gene Expression Lupinus / enzymology* Lupinus / genetics Lysine / metabolism Metabolome* Nitrogen / metabolism* Plant Proteins / genetics Plant Proteins / metabolism Plants, Genetically Modified Transgenes
IF 5.726
Times Cited 1
Arabidopsis / Cultured plant cells, genes pda07810, pda01165