RRC ID 59929
Author Abe K, Shimada A, Tayama S, Nishikawa H, Kaneko T, Tsuda S, Karaiwa A, Matsui T, Ishitani T, Takeda H.
Title Horizontal Boundary Cells, a Special Group of Somitic Cells, Play Crucial Roles in the Formation of Dorsoventral Compartments in Teleost Somite.
Journal Cell Rep
Abstract Establishment of robust gene expression boundary is crucial for creating elaborate morphology during development. However, mechanisms underlying boundary formation have been extensively studied only in a few model systems. We examined the establishment of zic1/zic4-expression boundary demarcating dorsoventral boundary of the entire trunk of medaka fish (Oryzias latipes) and identified a subgroup of dermomyotomal cells called horizontal boundary cells (HBCs) as crucial players for the boundary formation. Embryological and genetic analyses demonstrated that HBCs play crucial roles in the two major events of the process, i.e., refinement and maintenance. In the refinement, HBCs could serve as a chemical barrier against Wnts from the neural tube by expressing Hhip. At later stages, HBCs participate in the maintenance of the boundary by differentiating into the horizontal myoseptum physically inhibiting cell mixing across the boundary. These findings reveal the mechanisms underlying the dorsoventral boundary in the teleost trunk by specialized boundary cells.
Volume 27(3)
Pages 928-939.e4
Published 2019-4-16
DOI 10.1016/j.celrep.2019.03.068
PII S2211-1247(19)30398-5
PMID 30995487
MeSH Animals Animals, Genetically Modified / metabolism Body Patterning / genetics Cell Differentiation Chromosomes, Artificial, Bacterial / genetics Fish Proteins / genetics Fish Proteins / metabolism* Gene Expression Regulation, Developmental Oryzias / metabolism Somites / cytology Somites / metabolism* Transcription Factors / genetics Transcription Factors / metabolism* Zebrafish / metabolism
IF 8.109
Times Cited 0
Resource
Zebrafish Tg(6xTcf/LefBS-miniP:d2EGFP)