論文 - 詳細
| RRC ID | 59930 |
|---|---|
| 著者 | Munisso MC, Yamaoka T. |
| タイトル | Evolution of Phage Display Approaches to Select Highly Specific Hemocompatible Peptides. |
| ジャーナル | Tissue Eng Part C Methods |
| Abstract |
The ideal blood-contacting surface would support endothelial cell lining and suppress platelet adhesion, but, in synthetic biomaterials, these issues often conflict with each other. The reconciliation of this dichotomy may arise by modifying the biomaterial surfaces with "smart" peptides. Phage display is a powerful method for discovering unique peptides capable of binding to target molecules, but the selection of peptides binding to intact cells is an intricate process. In fact, the target molecules are often hindered by the extremely complex composition of cell membrane. In this work, the traditional phage display screening approach against endothelial progenitor cells (EPCs) was implemented with the introduction of (1) a negative selection step against platelets and (2) the target affinity scoring function phage binding index. The peptide candidates were used to modify an expanded polytetrafluoroethylene (ePTFE) surface to demonstrate that one of them not only has high affinity for EPCs but also simultaneously decreases thrombus formation. |
| 巻・号 | 25(5) |
| ページ | 288-295 |
| 公開日 | 2019-5-1 |
| DOI | 10.1089/ten.TEC.2018.0367 |
| PMID | 30854940 |
| MeSH | Amino Acid Sequence Animals Blood Coagulation / drug effects Cell Surface Display Techniques* Endothelial Progenitor Cells / cytology Endothelial Progenitor Cells / drug effects Materials Testing* Peptides / chemistry Peptides / pharmacology* Swine Thrombosis / pathology |
| IF | 3.508 |
| 引用数 | 2 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | News |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2 |