| RRC ID |
59931
|
| 著者 |
Dubiel M, De Coninck T, Osterne VJS, Verbeke I, Van Damme D, Smagghe G, Van Damme EJM.
|
| タイトル |
The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion.
|
| ジャーナル |
Int J Mol Sci
|
| Abstract |
Stress granules are cytoplasmic compartments, which serve as mRNA storage units during stress, therefore regulating translation. The Arabidopsis thaliana lectin ArathEULS3 has been widely described as a stress inducible gene. This study aimed to examine in detail the localization of ArathEULS3 lectin in normal and stressed cells. Colocalization experiments revealed that the nucleo-cytoplasmic lectin ArathEULS3 relocates to stress granules after stress. The ArathEULS3 sequence encodes a protein with a EUL lectin domain and an N-terminal domain with unknown structure and function. Bioinformatics analyses showed that the N-terminal domain sequence contains intrinsically disordered regions and likely does not exhibit a stable protein fold. Plasmolysis experiments indicated that ArathEULS3 also localizes to the apoplast, suggesting that this protein might follow an unconventional route for secretion. As part of our efforts we also investigated the interactome of ArathEULS3 and identified several putative interaction partners important for the protein translation process.
|
| 巻・号 |
21(5)
|
| 公開日 |
2020-2-28
|
| DOI |
10.3390/ijms21051659
|
| PII |
ijms21051659
|
| PMID |
32121292
|
| PMC |
PMC7084908
|
| MeSH |
Amino Acid Sequence
Arabidopsis / metabolism*
Arabidopsis Proteins / chemistry
Arabidopsis Proteins / metabolism*
Cell Nucleus / metabolism
Cytoplasmic Granules / metabolism*
Green Fluorescent Proteins / metabolism
Lectins / chemistry
Lectins / metabolism*
Protein Binding
Stress, Physiological*
|
| IF |
4.183
|
| 引用数 |
0
|
| リソース情報 |
| シロイヌナズナ / 植物培養細胞・遺伝子 |
pda02666 |
