RRC ID 59931
Author Dubiel M, De Coninck T, Osterne VJS, Verbeke I, Van Damme D, Smagghe G, Van Damme EJM.
Title The ArathEULS3 Lectin Ends up in Stress Granules and Can Follow an Unconventional Route for Secretion.
Journal Int J Mol Sci
Abstract Stress granules are cytoplasmic compartments, which serve as mRNA storage units during stress, therefore regulating translation. The Arabidopsis thaliana lectin ArathEULS3 has been widely described as a stress inducible gene. This study aimed to examine in detail the localization of ArathEULS3 lectin in normal and stressed cells. Colocalization experiments revealed that the nucleo-cytoplasmic lectin ArathEULS3 relocates to stress granules after stress. The ArathEULS3 sequence encodes a protein with a EUL lectin domain and an N-terminal domain with unknown structure and function. Bioinformatics analyses showed that the N-terminal domain sequence contains intrinsically disordered regions and likely does not exhibit a stable protein fold. Plasmolysis experiments indicated that ArathEULS3 also localizes to the apoplast, suggesting that this protein might follow an unconventional route for secretion. As part of our efforts we also investigated the interactome of ArathEULS3 and identified several putative interaction partners important for the protein translation process.
Volume 21(5)
Published 2020-2-28
DOI 10.3390/ijms21051659
PII ijms21051659
PMID 32121292
PMC PMC7084908
MeSH Amino Acid Sequence Arabidopsis / metabolism* Arabidopsis Proteins / chemistry Arabidopsis Proteins / metabolism* Cell Nucleus / metabolism Cytoplasmic Granules / metabolism* Green Fluorescent Proteins / metabolism Lectins / chemistry Lectins / metabolism* Protein Binding Stress, Physiological*
IF 4.183
Times Cited 0
Arabidopsis / Cultured plant cells, genes pda02666