| RRC ID |
59983
|
| 著者 |
Kanamaru R, Ohzawa H, Miyato H, Matsumoto S, Haruta H, Kurashina K, Saito S, Hosoya Y, Yamaguchi H, Yamashita H, Seto Y, Lefor AK, Sata N, Kitayama J.
|
| タイトル |
Low density neutrophils (LDN) in postoperative abdominal cavity assist the peritoneal recurrence through the production of neutrophil extracellular traps (NETs).
|
| ジャーナル |
Sci Rep
|
| Abstract |
Many types of immune cells appear in peritoneal cavity after abdominal surgery. In patients who underwent laparotomy due to gastric cancer, peritoneal lavages were obtained before and after surgical procedure. Cells were recovered from intermediate layer after Ficoll-Hypaque centrifugation and analyzed for phenotypes and functions, especially focused on low density neutrophils (LDN). The number of CD66b (+) LDN with mature phenotype was markedly elevated in postoperative as compared with preoperative lavages. Short term culture of the purified LDN produced many threadlike structures positive for SYTOX, nucleic acid staining, as well as histone and myeloperoxidase, suggesting the NETs formation. Human gastric cancer cells, MKN45, OCUM-1 and NUGC-4, were selectively attached on the NETs, which was totally abolished by the pretreatment of DNAse I. Intraperitoneal (IP) co-transfer of the LDN with MKN45 in nude mice strongly augments the metastasis formation on peritoneum, which was strongly suppressed by the following IP administration of DNAse I. Many NETs-like structures were detected on the surface of human omental tissue resected by gastrectomy. NETs on peritoneal surface can assist the clustering and growth of free tumor cells disseminated in abdomen. Disruption of the NETs by DNAse might be useful to prevent the peritoneal recurrence after abdominal surgery.
|
| 巻・号 |
8(1)
|
| ページ |
632
|
| 公開日 |
2018-1-12
|
| DOI |
10.1038/s41598-017-19091-2
|
| PII |
10.1038/s41598-017-19091-2
|
| PMID |
29330531
|
| PMC |
PMC5766579
|
| MeSH |
Animals
Antigens, CD / metabolism
Cell Adhesion Molecules / metabolism
Cell Culture Techniques
Cell Line, Tumor
Extracellular Traps / metabolism*
GPI-Linked Proteins / metabolism
Humans
Male
Mice
Mice, Nude
Neoplasm Recurrence, Local
Neutrophils / cytology
Neutrophils / immunology*
Peritoneal Lavage
Peritoneal Neoplasms / metabolism
Peritoneal Neoplasms / pathology*
Peritoneal Neoplasms / secondary*
Stomach Neoplasms / immunology
Stomach Neoplasms / metabolism
Stomach Neoplasms / surgery*
|
| IF |
3.998
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001)
NUGC-4(RCB1939) |
