RRC ID |
60016
|
著者 |
Sakai R, Tanano K, Ono T, Kitano M, Iida Y, Nakano K, Jimbo M.
|
タイトル |
Soritesidine, a Novel Proteinous Toxin from the Okinawan Marine Sponge Spongosorites sp.
|
ジャーナル |
Mar Drugs
|
Abstract |
A novel protein, soritesidine (SOR) with potent toxicity was isolated from the marine sponge Spongosorites sp. SOR exhibited wide range of toxicities over various organisms and cells including brine shrimp (Artemia salina) larvae, sea hare (Aplysia kurodai) eggs, mice, and cultured mammalian cells. Toxicities of SOR were extraordinary potent. It killed mice at 5 ng/mouse after intracerebroventricular (i.c.v.) injection, and brine shrimp and at 0.34 µg/mL. Cytotoxicity for cultured mammalian cancer cell lines against HeLa and L1210 cells were determined to be 0.062 and 12.11 ng/mL, respectively. The SOR-containing fraction cleaved plasmid DNA in a metal ion dependent manner showing genotoxicity of SOR. Purified SOR exhibited molecular weight of 108.7 kDa in MALDI-TOF MS data and isoelectric point of approximately 4.5. N-terminal amino acid sequence up to the 25th residue was determined by Edman degradation. Internal amino acid sequences for fifteen peptides isolated from the enzyme digest of SOR were also determined. None of those amino acid sequences showed similarity to existing proteins, suggesting that SOR is a new proteinous toxin.
|
巻・号 |
17(4)
|
公開日 |
2019-4-8
|
DOI |
10.3390/md17040216
|
PII |
md17040216
|
PMID |
30965587
|
PMC |
PMC6520796
|
MeSH |
Amino Acid Sequence
Animals
Aplysia / drug effects
Artemia / drug effects
Behavior, Animal / drug effects
Biological Assay / methods
Cell Line, Tumor
Humans
Japan
Larva / drug effects
Male
Marine Toxins / administration & dosage
Marine Toxins / chemistry
Marine Toxins / isolation & purification
Marine Toxins / toxicity*
Mice
Molecular Weight
Mutagenicity Tests / methods
Porifera*
|
IF |
3.772
|
引用数 |
0
|
リソース情報 |
ヒト・動物細胞 |
L1210 TGR4(RCB2844) |