| RRC ID |
60027
|
| 著者 |
Sakuda T, Kubo T, Johan MP, Furuta T, Sakaguchi T, Nakanishi M, Ochi M, Adachi N.
|
| タイトル |
Novel Near-Infrared Fluorescence-Guided Surgery With Vesicular Stomatitis Virus for Complete Surgical Resection of Osteosarcomas in Mice.
|
| ジャーナル |
J Orthop Res
|
| Abstract |
Attempts have been made to visualize tumor cells intraoperatively with fluorescence guidance. However, the clear demarcation and complete tumor resection have always been a challenging task. To address this, we have developed a novel fluorescence bioimaging system with vesicular stomatitis virus (VSV) incorporating Katushka, near-infrared fluorescent protein. VSV is tumor-specific owing to the deficiency of antiviral interferon signaling pathways in tumor cells. We aimed to evaluate the tumor specificity of the recombinant VSV-Katushka (rVSV-K) in osteosarcoma cells and to assess the feasibility of complete tumor resection by the rVSV-K fluorescence guidance. In in vitro experiments, mouse and human osteosarcoma cell lines and normal human mesenchymal stem cells were infected with rVSV-K and observed by fluorescence microscopy. Near-infrared fluorescence was observed only in osteosarcoma cells, even at a low-concentration of virus infections. In in vivo experiments, mouse osteosarcoma (LM8) cells were transplanted subcutaneously into the back of immune-competent mice to produce an osteosarcoma, which was then injected with rVSV-K. The areas emitting fluorescence were resected using a bioimaging system. The distance between the surgical and tumor margins of the fluorescence-guided resection with rVSV-K group was significantly larger than that of the non-guided resection groups. The local recurrence rate was significantly lower in the fluorescence-guided resection with rVSV-K group than in the non-guided resection groups. The distant metastasis rate and average survival rate were not significantly different between all groups. These results suggest that the rVSV-K is specific to osteosarcoma cells and enables complete tumor resection of osteosarcomas in mice. © 2019 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res.
|
| 巻・号 |
37(5)
|
| ページ |
1192-1201
|
| 公開日 |
2019-5-1
|
| DOI |
10.1002/jor.24277
|
| PMID |
30839125
|
| MeSH |
Animals
Fluorescent Dyes*
Humans
Male
Mice, Inbred C3H
Neoplasm Recurrence, Local
Neoplasms, Experimental / mortality
Neoplasms, Experimental / surgery*
Osteosarcoma / mortality
Osteosarcoma / surgery*
Vesiculovirus*
|
| IF |
2.728
|
| 引用数 |
0
|
| リソース情報 |
| ヒト・動物細胞 |
LM8(RCB1450) |
