| RRC ID |
60030
|
| Author |
Ota R, Ohkubo Y, Yamashita Y, Ogawa-Ohnishi M, Matsubayashi Y.
|
| Title |
Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis.
|
| Journal |
Nat Commun
|
| Abstract |
Plants modulate the efficiency of root nitrogen (N) acquisition in response to shoot N demand. However, molecular components directly involved in this shoot-to-root communication remain to be identified. Here, we show that phloem-mobile CEPD-like 2 (CEPDL2) polypeptide is upregulated in the leaf vasculature in response to decreased shoot N status and, after translocation to the roots, promotes high-affinity uptake and root-to-shoot transport of nitrate. Loss of CEPDL2 leads to a reduction in shoot nitrate content and plant biomass. CEPDL2 contributes to N acquisition cooperatively with CEPD1 and CEPD2 which mediate root N status, and the complete loss of all three proteins severely impairs N homeostasis in plants. Reciprocal grafting analysis provides conclusive evidence that the shoot CEPDL2/CEPD1/2 genotype defines the high-affinity nitrate uptake activity in root. Our results indicate that plants integrate shoot N status and root N status in leaves and systemically regulate the efficiency of root N acquisition.
|
| Volume |
11(1)
|
| Pages |
641
|
| Published |
2020-1-31
|
| DOI |
10.1038/s41467-020-14440-8
|
| PII |
10.1038/s41467-020-14440-8
|
| PMID |
32005881
|
| PMC |
PMC6994653
|
| MeSH |
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Biological Transport
Gene Expression Regulation, Plant
Glutaredoxins / genetics
Glutaredoxins / metabolism*
Nitrates / metabolism*
Plant Leaves / genetics
Plant Leaves / metabolism
Plant Roots / genetics
Plant Roots / metabolism*
Plant Shoots / genetics
Plant Shoots / metabolism*
|
| IF |
11.878
|
| Times Cited |
7
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pst10389, pst11057, pst18898 |
