RRC ID |
60045
|
著者 |
Gunawan F, Gentile A, Fukuda R, Tsedeke AT, Jiménez-Amilburu V, Ramadass R, Iida A, Sehara-Fujisawa A, Stainier DYR.
|
タイトル |
Focal adhesions are essential to drive zebrafish heart valve morphogenesis.
|
ジャーナル |
J Cell Biol
|
Abstract |
Elucidating the morphogenetic events that shape vertebrate heart valves, complex structures that prevent retrograde blood flow, is critical to understanding valvular development and aberrations. Here, we used the zebrafish atrioventricular (AV) valve to investigate these events in real time and at single-cell resolution. We report the initial events of collective migration of AV endocardial cells (ECs) into the extracellular matrix (ECM), and their subsequent rearrangements to form the leaflets. We functionally characterize integrin-based focal adhesions (FAs), critical mediators of cell-ECM interactions, during valve morphogenesis. Using transgenes to block FA signaling specifically in AV ECs as well as loss-of-function approaches, we show that FA signaling mediated by Integrin α5β1 and Talin1 promotes AV EC migration and overall shaping of the valve leaflets. Altogether, our investigation reveals the critical processes driving cardiac valve morphogenesis in vivo and establishes the zebrafish AV valve as a vertebrate model to study FA-regulated tissue morphogenesis.
|
巻・号 |
218(3)
|
ページ |
1039-1054
|
公開日 |
2019-3-4
|
DOI |
10.1083/jcb.201807175
|
PII |
jcb.201807175
|
PMID |
30635353
|
PMC |
PMC6400548
|
MeSH |
Animals
Cell Movement
Endocardium / embryology*
Extracellular Matrix / genetics
Extracellular Matrix / metabolism
Focal Adhesions / genetics
Focal Adhesions / metabolism*
Heart Valves / embryology*
Integrin alpha5beta1 / genetics
Integrin alpha5beta1 / metabolism
Organogenesis*
Signal Transduction*
Talin / genetics
Talin / metabolism
Zebrafish / embryology*
Zebrafish / genetics
Zebrafish Proteins / genetics
Zebrafish Proteins / metabolism
|
IF |
8.891
|
引用数 |
9
|
リソース情報 |
ゼブラフィッシュ |
itga5 kt451, UAS:GFP, UAS:RFP |