| RRC ID |
60090
|
| 著者 |
Antinucci P, Dumitrescu A, Deleuze C, Morley HJ, Leung K, Hagley T, Kubo F, Baier H, Bianco IH, Wyart C.
|
| タイトル |
A calibrated optogenetic toolbox of stable zebrafish opsin lines.
|
| ジャーナル |
Elife
|
| Abstract |
Optogenetic actuators with diverse spectral tuning, ion selectivity and kinetics are constantly being engineered providing powerful tools for controlling neural activity with subcellular resolution and millisecond precision. Achieving reliable and interpretable in vivo optogenetic manipulations requires reproducible actuator expression and calibration of photocurrents in target neurons. Here, we developed nine transgenic zebrafish lines for stable opsin expression and calibrated their efficacy in vivo. We first used high-throughput behavioural assays to compare opsin ability to elicit or silence neural activity. Next, we performed in vivo whole-cell electrophysiological recordings to quantify the amplitude and kinetics of photocurrents and test opsin ability to precisely control spiking. We observed substantial variation in efficacy, associated with differences in both opsin expression level and photocurrent characteristics, and identified conditions for optimal use of the most efficient opsins. Overall, our calibrated optogenetic toolkit will facilitate the design of controlled optogenetic circuit manipulations.
|
| 巻・号 |
9
|
| 公開日 |
2020-3-27
|
| DOI |
10.7554/eLife.54937
|
| PII |
54937
|
| PMID |
32216873
|
| PMC |
PMC7170653
|
| MeSH |
Animals
Animals, Genetically Modified
Calibration
Chlorides / metabolism
Escape Reaction
Motor Neurons / physiology
Opsins / genetics*
Optogenetics*
Proton Pumps / physiology
Rhodopsin / physiology
Trigeminal Ganglion / embryology
Zebrafish / embryology
|
| IF |
7.551
|
| 引用数 |
0
|
| リソース情報 |
| ゼブラフィッシュ |
Tg(chx10:Gal4), UAS:GFP |
