RRC ID 60096
Author Urasaki A, Morishita S, Naka K, Uozumi M, Abe K, Huang L, Watase E, Nakagawa O, Kawakami K, Matsui T, Bessho Y, Inagaki N.
Title Shootins mediate collective cell migration and organogenesis of the zebrafish posterior lateral line system.
Journal Sci Rep
Abstract The zebrafish sensory posterior lateral line is an excellent model system to study collective cell migration and organogenesis. Shootin1 is a cytoplasmic protein involved in neuronal polarization and axon guidance. Previous studies have shown that shootin1 couples actin filament retrograde flow with extracellular adhesive substrates at the leading edge of axonal growth cones, thereby producing mechanical force for the migration and guidance of axonal growth cones. However, the functions of shootin in peripheral cells remain unknown. Here we identified two novel shootin family members, shootin2 and shootin3. In zebrafish, shootin1 and shootin3 are expressed in the posterior lateral line primordium (PLLP) and neuromasts during embryonic development. A shootin1 mutant displayed a reduced speed of PLLP migration, while shootin1;shootin3 double mutation inhibited cell proliferation in the PLLP. Furthermore, our results suggest that shootin1 and shootin3 positively regulate the number of neuromasts and the number of cells in deposited neuromasts. Our study demonstrates that shootins mediate collective cell migration of the posterior lateral line primordium and formation of neuromasts in zebrafish.
Volume 9(1)
Pages 12156
Published 2019-8-21
DOI 10.1038/s41598-019-48585-4
PII 10.1038/s41598-019-48585-4
PMID 31434971
PMC PMC6704158
MeSH Actins / metabolism Animals Carrier Proteins / classification Carrier Proteins / genetics Carrier Proteins / metabolism* Cell Movement Embryonic Development Gene Editing Lateral Line System / metabolism* Microscopy, Fluorescence Neurons / physiology Organogenesis Phylogeny Protein Binding Zebrafish / growth & development* Zebrafish / metabolism Zebrafish Proteins / classification Zebrafish Proteins / genetics Zebrafish Proteins / metabolism*
IF 4.011
Times Cited 0