論文 - 詳細
RRC ID | 60119 |
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著者 | Saiki P, Kawano Y, Nakajima Y, Van Griensven LJLD, Miyazaki K. |
タイトル | Novel and Stable Dual-Color IL-6 and IL-10 Reporters Derived from RAW 264.7 for Anti-Inflammation Screening of Natural Products. |
ジャーナル | Int J Mol Sci |
Abstract |
Our previous study suggested that the interleukin (IL)-6 and IL-10 could serve as good biomarkers for chronic inflammatory disease. We previously established an IL-6 and IL-10 reporters assay that could examine reporter activity along with the reference gene in LPS-induced RAW 264.7 cells. In this study, we described new and stable RAW 264.7 derived dual-color IL-6/gapdh and IL-10/gapdh reporters. This assay allowed us to easily determine relative IL-6 and IL-10 levels with 96-well plate within one step. We evaluated the relative IL-6 and IL-10 levels in the LPS-induced stable cells testing 52 natural products by real-time bioluminescence monitoring and time-point determination using a microplate luminometer. The relative IL-6 and IL-6/IL-10 values decreased by the crude ethanol extracts from nutmeg and by 1'S-1'-acetoxychavicol from greater galangal using real-time bioluminescence monitoring. At the same time, the relative IL-10 was induced. The relative IL-6 and IL-6/IL-10 decreased by crude ethanol extracts from nutmeg and 1'S-1'-acetoxychavicol acetate at 6 h. Only crude ethanol extract from nutmeg induced IL-10 at 6 h. We suggested that the use of these stable cells by real-time monitoring could serve as a screening assay for anti-inflammatory activity and may be used to discover new drugs against chronic inflammatory disease. |
巻・号 | 20(18) |
公開日 | 2019-9-18 |
DOI | 10.3390/ijms20184620 |
PII | ijms20184620 |
PMID | 31540402 |
PMC | PMC6769898 |
MeSH | Animals Anti-Inflammatory Agents / pharmacology* Biological Products / pharmacology Biomarkers, Pharmacological / analysis Drug Evaluation, Preclinical / methods Inflammation / drug therapy Inflammation / immunology Interleukin-10 / analysis* Interleukin-10 / immunology Interleukin-6 / analysis* Interleukin-6 / immunology Lipopolysaccharides / immunology Luminescent Measurements / methods Macrophages / drug effects* Macrophages / immunology Mice RAW 264.7 Cells |
IF | 4.183 |
引用数 | 0 |
リソース情報 | |
ヒト・動物細胞 | RAW 264(RCB0535) |