RRC ID 60168
Author Mao W, Han Y, Wang X, Zhao X, Chi Z, Chi Z, Liu G.
Title A new engineered endo-inulinase with improved activity and thermostability: Application in the production of prebiotic fructo-oligosaccharides from inulin.
Journal Food Chem
Abstract To construct a high-performance engineered endo-inulinase for fructo-oligosaccharides (FOS) production from inulin, an inulin binding module (IBM) was fused into either N- or C-terminal of an endo-inulinase. After heterologous expression, purification and characterization, the C-terminal fusion one (Eninu-IBM) with better activity, thermostability and inulin binding ability was employed for high-temperature in situ inulin hydrolysis in a 10-L fermentor. During this process, Eninu-IBM was first efficiently produced by the yeast cells at 28 °C for 96 h, and subsequently 1600 g unsterilized inulin per liter fermentation liquor was directly supplemented into the bioreactor for FOS production at 60 °C for 2 h. Finally, high purity of FOS (91.4%) were obtained with FOS titer, yield and productivity of 717.3 g/L, 0.912 gFOS/gInulin and 358.6 g/L/h, respectively. The in vitro prebiotic assay indicated that the final FOS products with main polymerization degrees of 3-5 were preferably fermented by beneficial bifidobacteria and lactobacilli.
Volume 294
Pages 293-301
Published 2019-10-1
DOI 10.1016/j.foodchem.2019.05.062
PII S0308-8146(19)30857-X
PMID 31126466
MeSH Bioreactors Glycoside Hydrolases / genetics Glycoside Hydrolases / metabolism* Hydrogen-Ion Concentration Hydrolysis Inulin / metabolism* Kinetics Oligosaccharides / metabolism* Prebiotics Protein Stability Recombinant Proteins / biosynthesis Recombinant Proteins / isolation & purification Saccharomycetales / growth & development Saccharomycetales / metabolism Temperature
IF 6.306
Times Cited 4
General Microbes JCM7019