| RRC ID |
60208
|
| 著者 |
Li HZ, Bi QF, Yang K, Zheng BX, Pu Q, Cui L.
|
| タイトル |
D2O-Isotope-Labeling Approach to Probing Phosphate-Solubilizing Bacteria in Complex Soil Communities by Single-Cell Raman Spectroscopy.
|
| ジャーナル |
Anal Chem
|
| Abstract |
Increasing the bioavailability of immobilized phosphorus (P) in soil by phosphate-solubilizing bacteria (PSB) is an effective strategy for sustainable agronomic use of P and for mitigating the P crisis. Here, D2O isotope labeling combined with single-cell Raman spectroscopy (Raman-D2O) was developed as an efficient activity-based approach to characterizing the presence and activity of PSB in a culture-independent way. On the basis of the finding that PSB were significantly more active than non-PSB in the presence of insoluble P, a C-D Raman band from active assimilation of D2O-derived D was established as a biomarker for both inorganic-phosphate-solubilizing bacteria and organic-phosphate-solubilizing bacteria. C-D ratios (intensities of C-D bands as percentages of the intensities of both the C-D and C-H bands) were further established as semiquantitative indicators of P-releasing activities because of the consistency between the C-D ratio and the concentration of solubilized phosphate or acid phosphatase activity as measured by conventional bulk assays. By applying Raman imaging, single-cell Raman-D2O clearly discerned PSB in a mixed-soil bacterial culture and even in complex soil communities. Remarkable heterogeneity of microbial activity, ranging from 2 to 30% (close to that in medium without P and that in medium with sufficient soluble P, respectively), was revealed at the single-cell level and clearly illustrated the subpopulation of soil bacteria active in solubilizing P. This work not only enables probing PSB and their P-releasing activities but also opens a window to explore more diverse microbial resources when obtaining related isotope-labeled substrates is prohibitive.
|
| 巻・号 |
91(3)
|
| ページ |
2239-2246
|
| 公開日 |
2019-2-5
|
| DOI |
10.1021/acs.analchem.8b04820
|
| PMID |
30608659
|
| MeSH |
Bacteria / isolation & purification*
Bacteria / metabolism
Biomarkers / analysis
Deuterium / analysis
Deuterium Oxide / metabolism*
Isotope Labeling
Organophosphates / metabolism*
Phosphates / metabolism*
Soil Microbiology*
Spectrum Analysis, Raman
|
| IF |
6.35
|
| 引用数 |
6
|
| リソース情報 |
| 一般微生物 |
JCM21718 |
