RRC ID 60231
著者 Castro JF, Nouioui I, Asenjo JA, Andrews B, Bull AT, Goodfellow M.
タイトル New genus-specific primers for PCR identification of Rubrobacter strains.
ジャーナル Antonie Van Leeuwenhoek
Abstract A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
巻・号 112(12)
ページ 1863-1874
公開日 2019-12-1
DOI 10.1007/s10482-019-01314-3
PII 10.1007/s10482-019-01314-3
PMID 31407134
PMC PMC6834744
MeSH Actinobacteria / classification Actinobacteria / genetics Actinobacteria / isolation & purification* DNA Primers / genetics DNA, Bacterial / genetics DNA, Ribosomal / genetics Molecular Diagnostic Techniques / methods* Polymerase Chain Reaction / methods* RNA, Ribosomal, 16S / genetics Sensitivity and Specificity Soil Microbiology South America
IF 1.934
引用数 0
リソース情報
一般微生物 JCM19154 JCM19155 JCM2153 JCM12932 JCM11954 JCM11494 JCM16550 JCM19086 JCM13025 JCM31078 JCM14923 JCM31079