RRC ID |
60231
|
著者 |
Castro JF, Nouioui I, Asenjo JA, Andrews B, Bull AT, Goodfellow M.
|
タイトル |
New genus-specific primers for PCR identification of Rubrobacter strains.
|
ジャーナル |
Antonie Van Leeuwenhoek
|
Abstract |
A set of oligonucleotide primers, Rubro223f and Rubro454r, were found to amplify a 267 nucleotide sequence of 16S rRNA genes of Rubrobacter type strains. The primers distinguished members of this genus from other deeply-rooted actinobacterial lineages corresponding to the genera Conexibacter, Gaiella, Parviterribacter, Patulibacter, Solirubrobacter and Thermoleophilum of the class Thermoleophilia. Amplification of DNA bands of about 267 nucleotides were generated from environmental DNA extracted from soil samples taken from two locations in the Atacama Desert. Sequencing of a DNA library prepared from the bands showed that all of the clones fell within the evolutionary radiation occupied by the genus Rubrobacter. Most of the clones were assigned to two lineages that were well separated from phyletic lines composed of Rubrobacter type strains. It can be concluded that primers Rubro223f and Rubro454r are specific for the genus Rubrobacter and can be used to detect the presence and abundance of members of this genus in the Atacama Desert and other biomes.
|
巻・号 |
112(12)
|
ページ |
1863-1874
|
公開日 |
2019-12-1
|
DOI |
10.1007/s10482-019-01314-3
|
PII |
10.1007/s10482-019-01314-3
|
PMID |
31407134
|
PMC |
PMC6834744
|
MeSH |
Actinobacteria / classification
Actinobacteria / genetics
Actinobacteria / isolation & purification*
DNA Primers / genetics
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Molecular Diagnostic Techniques / methods*
Polymerase Chain Reaction / methods*
RNA, Ribosomal, 16S / genetics
Sensitivity and Specificity
Soil Microbiology
South America
|
IF |
1.934
|
引用数 |
0
|
リソース情報 |
一般微生物 |
JCM19154
JCM19155
JCM2153
JCM12932
JCM11954
JCM11494
JCM16550
JCM19086
JCM13025
JCM31078
JCM14923
JCM31079 |