| RRC ID |
60232
|
| 著者 |
Sato Y, Kimura H.
|
| タイトル |
Temperature-dependent expression of different guanine-plus-cytosine content 16S rRNA genes in Haloarcula strains of the class Halobacteria.
|
| ジャーナル |
Antonie Van Leeuwenhoek
|
| Abstract |
Haloarcula strains, which are halophilic archaea, harbour two to three copies of 16S rRNA genes (rrsA, rrsB and rrsC) in their genomes. While rrsB and rrsC (rrsBC) show almost identical sequences, rrsA shows 4-6% sequence difference and 1-3% guanine-plus-cytosine content (PGC) difference compared to rrsBC. Based on the strong correlation between the PGC of 16S rRNA genes and the growth temperatures of the prokaryotes, we hypothesised that high-PGCrrsA and low-PGCrrsBC are expressed at high and low temperatures, respectively. To verify the hypothesis, we performed sequence analyses and expression surveys of each 16S rRNA gene in eight Haloarcula strains. The secondary structure prediction of the 16S rRNA via computer simulation showed that the structural stability of 16S rRNAs transcribed from rrsA was higher than that of 16S rRNAs transcribed from rrsBC. We measured expression levels of rrsA and rrsBC under various temperature conditions by reverse-transcriptase quantitative PCR. The expression ratio of high-PGCrrsA to low-PGCrrsBC increased with cultivation temperatures in seven of eight Haloarcula strains. Our results suggest that the transcription of high-PGCrrsA and low-PGCrrsBC may be regulated in response to environmental temperature, and that 16S rRNAs transcribed from high-PGCrrsA function under high temperature conditions close to the maximum growth temperature.
|
| 巻・号 |
112(2)
|
| ページ |
187-201
|
| 公開日 |
2019-2-1
|
| DOI |
10.1007/s10482-018-1144-3
|
| PII |
10.1007/s10482-018-1144-3
|
| PMID |
30128892
|
| PMC |
PMC6373231
|
| MeSH |
Base Composition*
Computer Simulation
Cytosine / analysis
Cytosine / metabolism
DNA, Archaeal / genetics*
DNA, Archaeal / metabolism
Guanine / analysis
Guanine / metabolism
Haloarcula / classification
Haloarcula / genetics*
Haloarcula / isolation & purification
Haloarcula / metabolism
Phylogeny
RNA, Ribosomal, 16S / genetics*
Temperature
|
| IF |
1.934
|
| 引用数 |
1
|
| リソース情報 |
| 一般微生物 |
JCM30477
JCM9737
JCM11048
JCM8877
JCM13557
JCM7785
JCM8911
JCM8912 |
