論文 - 詳細
| RRC ID | 60233 |
|---|---|
| 著者 | Kuncharoen N, Kudo T, Ohkuma M, Tanasupawat S. |
| タイトル | Micromonospora azadirachtae sp. nov., isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton. |
| ジャーナル | Antonie Van Leeuwenhoek |
| Abstract |
A Gram-stain positive actinomycete, strain AZ1-19T, isolated from roots of Azadirachta indica A. Juss. var. siamensis Valeton, collected from Chachoengsao province, Thailand, was characterised taxonomically by using a polyphasic approach. Strain AZ1-19T was found to have characteristics consistent with those of members of the genus Micromonospora. The cell wall peptidoglycan of the strain was found to contain meso-diaminopimelic acid. The predominant phospholipids were identified as diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The characteristic whole-cell sugars were identified as glucose, xylose, galactose and mannose. The major menaquinones were found to be MK-9(H6), MK-10(H6) and MK-10(H8), and the major cellular fatty acids were identified as iso-C16:0, iso-C15:0, anteiso-C15:0 and anteiso-C17:0. Comparative analysis of 16S rRNA gene sequences revealed that strain AZ1-19T is closely related to Micromonospora costi CS1-12T (98.75% similarity), Micromonospora avicenniae 268506T (98.75%), Micromonospora haikouensis 232617T (98.68%) and Micromonospora siamensis TT2-4T (98.61%), whilst the corresponding phylogenetic analysis based on partial gyrase subunit B (gyrB) gene sequences indicated that strain AZ1-19T forms a clade with M. avicenniae 268506T with a high bootstrap value. The DNA G + C content was determined to be 69.8 mol%. Moreover, a combination of DNA-DNA relatedness values and some phenotypic and chemotaxonomic properties indicated that the strain could be distinguished from closely related species. Therefore, it is considered that strain AZ1-19T represents a novel Micromonospora species for which the name Micromonospora azadirachtae sp. nov. is proposed. The type strain is AZ1-19T (= KCTC 39941T = NBRC 112784T = JCM 32148T = TISTR 2559T). |
| 巻・号 | 112(2) |
| ページ | 253-262 |
| 公開日 | 2019-2-1 |
| DOI | 10.1007/s10482-018-1152-3 |
| PII | 10.1007/s10482-018-1152-3 |
| PMID | 30167912 |
| MeSH | Azadirachta / microbiology* Bacterial Typing Techniques Base Composition DNA, Bacterial / genetics Diaminopimelic Acid / metabolism Fatty Acids / metabolism Micromonospora / classification Micromonospora / genetics Micromonospora / isolation & purification* Micromonospora / metabolism Phylogeny Plant Roots / microbiology* RNA, Ribosomal, 16S / genetics Soil Microbiology Thailand |
| IF | 1.934 |
| 引用数 | 4 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM32148 JCM31034 JCM31036 JCM12769 |