RRC ID 60238
著者 Shiobara T, Nakajima Y, Maeda K, Akasaka M, Kitou Y, Kanamaru K, Ohsato S, Kobayashi T, Nishiuchi T, Kimura M.
タイトル Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis.
ジャーナル Antonie Van Leeuwenhoek
Abstract Nitrogen sources in media have a significant impact on the onset of secondary metabolism in fungi. For transcriptional activation of many nitrogen catabolic genes, an AreA transcription factor is indispensable. This also holds true for Fusarium graminearum that produces trichothecenes, an important group of mycotoxin, in axenic culture. Despite the presence of numerous consensus AreA-binding sites in the promoters of Tri genes in the trichothecene cluster core region, the effect of medium amino acids on trichothecene biosynthesis is poorly understood. In this study, we examined the effect of certain amino acids, which were predicted to activate AreA function and increase Tri gene transcription, on trichothecene production in liquid culture. By frequent monitoring and adjustments in the pH of the culture medium, including replacement of the spent medium with fresh medium, we demonstrate the suppressive effects of the amino acids, used as the sole nitrogen source, on trichothecene biosynthesis. When the medium pH was maintained at 4.0, Gly, L-Ser, and L-Thr suppressed trichothecene production by F. graminearum. Enhanced trichothecene-inducing effects were observed when the medium pH was 3.5, with only L-Thr suppressing trichothecene synthesis.
巻・号 112(3)
ページ 471-478
公開日 2019-3-1
DOI 10.1007/s10482-018-1172-z
PII 10.1007/s10482-018-1172-z
PMID 30267234
MeSH Amino Acids / metabolism* Culture Media / chemistry Fusarium / growth & development Fusarium / metabolism* Gene Expression Regulation, Enzymologic / drug effects* Gene Expression Regulation, Fungal / drug effects* Hydrogen-Ion Concentration Trichothecenes / biosynthesis*
IF 1.934
引用数 2
リソース情報
一般微生物 JCM9873