| RRC ID |
60263
|
| 著者 |
Azam AH, Kadoi K, Miyanaga K, Usui M, Tamura Y, Cui L, Tanji Y.
|
| タイトル |
Analysis host-recognition mechanism of staphylococcal kayvirus ɸSA039 reveals a novel strategy that protects Staphylococcus aureus against infection by Staphylococcus pseudintermedius Siphoviridae phages.
|
| ジャーナル |
Appl Microbiol Biotechnol
|
| Abstract |
Following the emergence of antibiotic-resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and methicillin-resistant Staphylococcus pseudintermedius (MRSP), phage therapy has attracted significant attention as an alternative to antibiotic treatment. Bacteriophages belonging to kayvirus (previously known as Twort-like phages) have broad host range and are strictly lytic in Staphylococcus spp. Previous work revealed that kayvirus ɸSA039 has a host-recognition mechanism distinct from those of other known kayviruses: most of kayviruses use the backbone of wall teichoic acid (WTA) as their receptor; by contrast, ɸSA039 uses the β-N-acetylglucosamine (β-GlcNAc) residue in WTA. In this study, we found that ɸSA039 could switch its receptor to be able to infect S. aureus lacking the β-GlcNAc residue by acquiring a spontaneous mutation in open reading frame (ORF) 100 and ORF102. Moreover, ɸSA039 could infect S. pseudintermedius, which has a different WTA structure than S. aureus. By comparison, with newly isolated S. pseudintermedius-specific phage (SP phages), we determined that glycosylation in WTA of S. pseudintermedius is essential for adsorption of SP phages, but not ɸSA039. Finally, we describe a novel strategy of S. aureus which protects the bacteria from infection of SP phages. Notably, glycosylation of ribitol phosphate (RboP) WTA by TarM or/and TarS prevents infection of S. aureus by SP phages. These findings could help to establish a new strategy for the treatment of S. aureus and S. pseudintermedius infection, as well as provide valuable insights into the biology of phage-host interactions.
|
| 巻・号 |
103(16)
|
| ページ |
6809-6823
|
| 公開日 |
2019-8-1
|
| DOI |
10.1007/s00253-019-09940-7
|
| PII |
10.1007/s00253-019-09940-7
|
| PMID |
31236618
|
| MeSH |
Receptors, Virus / metabolism
Staphylococcus / virology*
Staphylococcus Phages / physiology*
Teichoic Acids / metabolism
Viral Interference*
Virus Attachment*
|
| IF |
3.67
|
| 引用数 |
4
|
| リソース情報 |
| 一般微生物 |
JCM20142 |
