| RRC ID |
60276
|
| 著者 |
Kim YJ, Perumalsamy H, Markus J, Balusamy SR, Wang C, Ho Kang S, Lee S, Park SY, Kim S, Castro-Aceituno V, Kim SH, Yang DC.
|
| タイトル |
Development of Lactobacillus kimchicus DCY51T-mediated gold nanoparticles for delivery of ginsenoside compound K: in vitro photothermal effects and apoptosis detection in cancer cells.
|
| ジャーナル |
Artif Cells Nanomed Biotechnol
|
| Abstract |
We report a non-covalent loading of ginsenoside compound K (CK) onto our previously reported gold nanoparticles (DCY51T-AuCKNps) through one-pot biosynthesis using a probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi. The ginsenoside-loaded gold nanoparticles were characterized by various analytical and spectroscopic techniques such as field emission transmission electron microscopy (FE-TEM), energy-dispersive X-ray (EDX) spectroscopy, elemental mapping, X-ray powder diffraction (XRD), selected area electron diffraction (SAED), Fourier-transform infrared (FTIR) spectroscopy and dynamic light scattering (DLS). Furthermore, drug loading was also determined by liquid chromatography-mass spectrometry (LC-MS). In addition, DCY51T-AuNps and DCY51T-AuCKNps were resistant to aggregation caused by pH variation or a high ionic strength environment. Cell-based study confirmed that DCY51T-AuCKNps exhibited slightly higher cytotoxicity compared to ginsenoside CK treatment in A549 cells (human lung adenocarcinoma cell line) and HT29 (human colorectal adenocarcinoma cell line). Upon laser treatment, DCY51T-AuCKNps showed enhanced cell apoptosis in A549, HT29 and AGS cells (human stomach gastric adenocarcinoma cell line) compared with only DCY51T-AuCKNps treated cells. In conclusion, this preliminary study identified that DCY51T-AuCKNps act as a potent photothermal therapy agents with synergistic chemotherapeutic effects for the treatment of cancer.
|
| 巻・号 |
47(1)
|
| ページ |
30-44
|
| 公開日 |
2019-12-1
|
| DOI |
10.1080/21691401.2018.1541900
|
| PMID |
30663395
|
| MeSH |
Animals
Apoptosis / drug effects*
Cell Line, Tumor
Drug Carriers / chemistry
Drug Carriers / metabolism
Ginsenosides / chemistry
Ginsenosides / pharmacology*
Gold / chemistry
Gold / metabolism*
Humans
Lactobacillus / metabolism*
Luminescent Measurements
Metal Nanoparticles*
Mice
Particle Size
Phototherapy*
Temperature*
|
| IF |
4.462
|
| 引用数 |
6
|
| リソース情報 |
| 一般微生物 |
JCM15530 |
