論文 - 詳細
| RRC ID | 60276 |
|---|---|
| 著者 | Kim YJ, Perumalsamy H, Markus J, Balusamy SR, Wang C, Ho Kang S, Lee S, Park SY, Kim S, Castro-Aceituno V, Kim SH, Yang DC. |
| タイトル | Development of Lactobacillus kimchicus DCY51T-mediated gold nanoparticles for delivery of ginsenoside compound K: in vitro photothermal effects and apoptosis detection in cancer cells. |
| ジャーナル | Artif Cells Nanomed Biotechnol |
| Abstract |
We report a non-covalent loading of ginsenoside compound K (CK) onto our previously reported gold nanoparticles (DCY51T-AuCKNps) through one-pot biosynthesis using a probiotic Lactobacillus kimchicus DCY51T isolated from Korean kimchi. The ginsenoside-loaded gold nanoparticles were characterized by various analytical and spectroscopic techniques such as field emission transmission electron microscopy (FE-TEM), energy-dispersive X-ray (EDX) spectroscopy, elemental mapping, X-ray powder diffraction (XRD), selected area electron diffraction (SAED), Fourier-transform infrared (FTIR) spectroscopy and dynamic light scattering (DLS). Furthermore, drug loading was also determined by liquid chromatography-mass spectrometry (LC-MS). In addition, DCY51T-AuNps and DCY51T-AuCKNps were resistant to aggregation caused by pH variation or a high ionic strength environment. Cell-based study confirmed that DCY51T-AuCKNps exhibited slightly higher cytotoxicity compared to ginsenoside CK treatment in A549 cells (human lung adenocarcinoma cell line) and HT29 (human colorectal adenocarcinoma cell line). Upon laser treatment, DCY51T-AuCKNps showed enhanced cell apoptosis in A549, HT29 and AGS cells (human stomach gastric adenocarcinoma cell line) compared with only DCY51T-AuCKNps treated cells. In conclusion, this preliminary study identified that DCY51T-AuCKNps act as a potent photothermal therapy agents with synergistic chemotherapeutic effects for the treatment of cancer. |
| 巻・号 | 47(1) |
| ページ | 30-44 |
| 公開日 | 2019-12-1 |
| DOI | 10.1080/21691401.2018.1541900 |
| PMID | 30663395 |
| MeSH | Animals Apoptosis / drug effects* Cell Line, Tumor Drug Carriers / chemistry Drug Carriers / metabolism Ginsenosides / chemistry Ginsenosides / pharmacology* Gold / chemistry Gold / metabolism* Humans Lactobacillus / metabolism* Luminescent Measurements Metal Nanoparticles* Mice Particle Size Phototherapy* Temperature* |
| IF | 4.462 |
| 引用数 | 6 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM15530 |