RRC ID 60279
Author Tanno H, Fujii T, Ose R, Hirano K, Tochio T, Endo A.
Title Characterization of fructooligosaccharide-degrading enzymes in human commensal Bifidobacterium longum and Anaerostipes caccae.
Journal Biochem Biophys Res Commun
Abstract Kestose and nystose are short chain fructooligosaccharides (scFOSs) with degrees of polymerization of 3 and 4, respectively. A previous study revealed that these scFOSs have different growth stimulation properties against two human commensals, i.e. Bifidobacterium longum subsp. longum and butyrogenic Anaerostipes caccae. The present study characterized genes involved in FOS metabolism in these organisms. A. caccae possesses a single gene cluster consisting of four genes, including a gene encoding the putative FOS degradation enzyme sucrose-6-phosphate hydrolase (S6PH). B. longum possesses two gene clusters consisting of three genes each, including genes encoding β-fructofuranosidase (CscA) and sucrose phosphorylase (ScrP). In A. caccae, the genes were highly transcribed in cells cultured with sucrose or kestose but poorly in cells cultured with glucose or nystose. Heterologously expressed S6PH degraded sucrose and kestose but not nystose. In B. longum, transcription of the genes was high in cells cultured with sucrose or kestose but was poor or not detected in cells cultured with glucose or nystose. Heterologously expressed CscA degraded sucrose, kestose and nystose, but ScrP degraded only sucrose. These data suggested that the different growth stimulation activities of kestose and nystose are due to different substrate specificities of FOS degradation enzymes in the organisms and/or induction activity of the genes in the two scFOSs. This is the first study characterizing the FOS metabolism at the transcriptional level and substrate-specificity of the degradation enzyme in butyrogenic human gut anaerobes.
Volume 518(2)
Pages 294-298
Published 2019-10-15
DOI 10.1016/j.bbrc.2019.08.049
PII S0006-291X(19)31566-9
PMID 31420164
MeSH Bifidobacterium longum / enzymology* Bifidobacterium longum / genetics Bifidobacterium longum / metabolism Clostridiales / enzymology* Clostridiales / genetics Clostridiales / metabolism Genes, Bacterial Glucosyltransferases / genetics Glucosyltransferases / metabolism Humans Multigene Family Oligosaccharides / metabolism* Substrate Specificity beta-Fructofuranosidase / genetics beta-Fructofuranosidase / metabolism
IF 2.705
Times Cited 1
General Microbes JCM13470 JCM1217