論文 - 詳細
| RRC ID | 60286 |
|---|---|
| 著者 | Fujimori K, Fujii S, Lisdiana L, Wakai S, Yagi H, Sambongi Y. |
| タイトル | Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
Deep-sea Shewanella violacea 5'-nucleotidase (SVNTase) activity exhibited higher NaCl tolerance than that of a shallow-sea Shewanella amazonensis homologue (SANTase), the sequence identity between them being 70.4%. Here, SVNTase exhibited higher activity than SANTase with various inorganic salts, similar to the difference in their NaCl tolerance. In contrast, SVNTase activity decreased with various organic solvents, while SANTase activity was retained with the same concentrations of the solvents. Therefore, SVNTase is more robust than SANTase with inorganic salts, but more vulnerable with organic solvents. As to protein stability, SANTase was more stable against organic solvents and heat than SVNTase, which correlated with the differences in their enzymatic activities. We also found that SANTase retained higher activity for three weeks than SVNTase did in the presence of glycerol. These findings will facilitate further application of these enzymes as appropriate biological catalysts under various harsh conditions. Abbreviations: NTase: 5'-nucleotidase; SANTase: Shewanella amazonensis 5'-nucleotidase; SVNTase: Shewanella violacea 5'-nucleotidase; CD: circular dichroism. |
| 巻・号 | 83(6) |
| ページ | 1085-1093 |
| 公開日 | 2019-6-1 |
| DOI | 10.1080/09168451.2019.1578641 |
| PMID | 30764715 |
| MeSH | 5'-Nucleotidase / chemistry 5'-Nucleotidase / metabolism* Adenosine Triphosphatases / metabolism Biocatalysis Catalytic Domain Circular Dichroism Enzyme Stability Hot Temperature Inorganic Chemicals / chemistry Organic Chemicals / chemistry Protein Conformation Salt Tolerance Seawater / microbiology* Shewanella / enzymology* Shewanella / physiology Solvents / chemistry |
| IF | 1.297 |
| 引用数 | 0 |
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 一般微生物 | JCM10179 |