RRC ID 60332
Author Jang WJ, Lee JM, Hasan MT, Lee BJ, Lim SG, Kong IS.
Title Effects of probiotic supplementation of a plant-based protein diet on intestinal microbial diversity, digestive enzyme activity, intestinal structure, and immunity in olive flounder (Paralichthys olivaceus).
Journal Fish Shellfish Immunol
Abstract The aim of this study was to investigate the effects of intestinal microbial manipulation by dietary probiotic supplementation on digestive enzyme activity, immune-related gene transcription, intestinal structure alteration, and viability against pathogenic challenge in olive flounder. Similar-sized flounders (14.92 ± 0.21 g) were divided into three groups and supplemented with a control (without probiotic) or 1 × 108 CFU/g diet of each of Bacillus sp. SJ-10 (ProB) and Lactobacillus plantarum (ProL) for eight weeks. At the end of the feeding trial, the estimated intestinal microbial richness (Chao1) and diversity (Shannon) demonstrated a significant (P < 0.05) abundance in the ProB group (484.80 ± 88.75, 5.08 ± 0.17) compared to the ProL (285.32 ± 17.78, 4.54 ± 0.09) and control groups (263.23 ± 20.20, 4.30 ± 0.20). A similar alteration phenomenon was also found at the phylum level, with a higher abundance of Proteobacteria, Actinobacteria, and Acidobacteria. Trypsin and lipase activities were elevated in both the ProB and ProL groups compared to the control, but amylase was only higher in the ProB group. The expression levels of pro-inflammatory cytokines, such as tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 were significantly higher in the ProB group than in the other two groups. There was a significant increase in transcription of IL-10 in both the ProB and ProL groups compared to the control. The length of villi and microvilli of probiotic-fed olive flounder was increased but was not significantly different from the control group. In an in vivo challenge experiment with Streptococcus iniae (1 × 108 CFU/mL), the survival rates of the ProB and ProL groups were 29.17% and 12.50%, respectively, when control mortality reached 100%. Therefore, intestinal microbiota manipulation by probiotic supplementation increased the richness of the bacterial population, digestive enzyme activity, intestinal immune gene transcription, and infectious disease protection in olive flounder.
Volume 92
Pages 719-727
Published 2019-9-1
DOI 10.1016/j.fsi.2019.06.056
PII S1050-4648(19)30703-X
PMID 31271838
MeSH Animal Feed / analysis Animal Nutritional Physiological Phenomena / drug effects Animals Diet / veterinary Digestion / drug effects* Flatfishes / anatomy & histology Flatfishes / microbiology Flatfishes / physiology* Gastrointestinal Microbiome / drug effects* Immunity, Innate / drug effects* Intestines / anatomy & histology Intestines / drug effects Intestines / enzymology Intestines / microbiology Plant Proteins, Dietary / administration & dosage* Probiotics / administration & dosage Probiotics / metabolism*
IF 3.298
Times Cited 6
Resource
General Microbes JCM15709