| RRC ID |
60350
|
| 著者 |
Ito T, Sekizuka T, Kishi N, Yamashita A, Kuroda M.
|
| タイトル |
Conventional culture methods with commercially available media unveil the presence of novel culturable bacteria.
|
| ジャーナル |
Gut Microbes
|
| Abstract |
Recent metagenomic analysis has revealed that our gut microbiota plays an important role in not only the maintenance of our health but also various diseases such as obesity, diabetes, inflammatory bowel disease, and allergy. However, most intestinal bacteria are considered 'unculturable' bacteria, and their functions remain unknown. Although culture-independent genomic approaches have enabled us to gain insight into their potential roles, culture-based approaches are still required to understand their characteristic features and phenotypes. To date, various culturing methods have been attempted to obtain these 'unculturable' bacteria, but most such methods require advanced techniques. Here, we have tried to isolate possible unculturable bacteria from a healthy Japanese individual by using commercially available media. A 16S rRNA (ribosomal RNA) gene metagenomic analysis revealed that each culture medium showed bacterial growth depending on its selective features and a possibility of the presence of novel bacterial species. Whole genome sequencing of these candidate strains suggested the isolation of 8 novel bacterial species classified in the Actinobacteria and Firmicutes phyla. Our approach indicates that a number of intestinal bacteria hitherto considered unculturable are potentially culturable and can be cultured on commercially available media. We have obtained novel gut bacteria from a healthy Japanese individual using a combination of comprehensive genomics and conventional culturing methods. We would expect that the discovery of such novel bacteria could illuminate pivotal roles for the gut microbiota in association with human health.
|
| 巻・号 |
10(1)
|
| ページ |
77-91
|
| 公開日 |
2019-1-1
|
| DOI |
10.1080/19490976.2018.1491265
|
| PMID |
30118379
|
| PMC |
PMC6363062
|
| MeSH |
Bacteria / classification
Bacteria / genetics
Bacteria / growth & development*
Bacteria / isolation & purification*
Bacteriological Techniques*
Culture Media
Feces / microbiology
Gastrointestinal Microbiome / genetics
Gastrointestinal Microbiome / physiology*
Genome, Bacterial / genetics
Humans
Metagenomics
Phylogeny
RNA, Ribosomal, 16S / genetics
Sequence Analysis, DNA
|
| IF |
7.74
|
| 引用数 |
7
|
| リソース情報 |
| 一般微生物 |
JCM32521
JCM32522
JCM32523
JCM32524
JCM32525
JCM32526
JCM32527
JCM32528 |
